7MNP

Crystal Structure of the ZnF2 of Nucleoporin NUP358/RanBP2 in complex with Ran-GDP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	GTP-binding nuclear protein Ran	polymer	217	24483.1	2	UniProt (P62826) Pfam (PF00071)	Homo sapiens (Human)	Androgen receptor-associated protein 24,GTPase Ran,Ras-like protein TC4,Ras-related nuclear protein
2	B, D (B, D)	E3 SUMO-protein ligase RanBP2	polymer	42	4610.3	2	UniProt (P49792) Pfam (PF00641)	Homo sapiens (Human)	358 kDa nucleoporin,Nuclear pore complex protein Nup358,Nucleoporin Nup358,Ran-binding protein 2,RanBP2,p270
3	E, H (A, C)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP)
4	F, I (A, C)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	G, J (B, D)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
6	K, L, M, N (A, B, C, D)	water	water		18.0	266	Chemie (HOH)

Sequence modifications

A, C: 1 - 216 (UniProt: P62826)

PDB	External Database	Details
Ser 0	-	expression tag
Ser 35	Phe 35	engineered mutation

B, D: 1407 - 1443 (UniProt: P49792)

PDB	External Database	Details
Gly 1402	-	expression tag
Pro 1403	-	expression tag
Leu 1404	-	expression tag
Gly 1405	-	expression tag
Ser 1406	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	58186.9
Non-Polymers*	Number of molecules	6
	Total formula weight	1065.8
All*	Total formula weight	59252.7

*Water molecules are not included.