7MM0

Cryo-EM structure of SARS-CoV-2 spike in complex with neutralizing antibody B1-182.1 that targets the receptor-binding domain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	H	B1-182.1 Fab heavy chain	polymer	228	24285.3	1		Homo sapiens
2	L	B1-182.1 Fab light chain	polymer	215	23536.0	1		Homo sapiens
3	A, B, C	Spike glycoprotein	polymer	1195	132334.5	3	UniProt (P0DTC2) Pfam (PF16451) Pfam (PF09408) Pfam (PF19209) Pfam (PF01601) In PDB	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV SARS-CoV-2)	S glycoprotein,E2,Peplomer protein
4	D, E, F, G, I...	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	13	In PDB GlyTouCan (G42666HT)
5	O	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)
6	A, B, C	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	27	Chemie (NAG)

Sequence modifications

A, B, C: 14 - 1208 (UniProt: P0DTC2)

PDB	External Database	Details
Gly 682	Arg 682	engineered mutation
Ser 683	Arg 683	engineered mutation
Ser 685	Arg 685	engineered mutation
Pro 817	Phe 817	engineered mutation
Pro 892	Ala 892	engineered mutation
Pro 899	Ala 899	engineered mutation
Pro 942	Ala 942	engineered mutation
Pro 986	Lys 986	engineered mutation
Pro 987	Val 987	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	444824.7
Branched	Number of molecules	14
	Total formula weight	6103.8
Non-Polymers*	Number of molecules	27
	Total formula weight	5972.6
All*	Total formula weight	456901.1

*Water molecules are not included.