7MKJ
Cryo-EM structure of Escherichia coli RNA polymerase bound to T7A1 promoter DNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, I (G, H, R) | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 3 | UniProt (A0A073G207) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) UniProt (by SIFTS) (P0A7Z4) | Escherichia coli | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | C (I) | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V4) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) UniProt (by SIFTS) (P0A8V2) | Escherichia coli | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 3 | D (J) | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155366.8 | 1 | UniProt (A0A4S1NBU2) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P0A8T7) | Escherichia coli | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 4 | E (K) | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (P0A802) Pfam (PF01192) UniProt (by SIFTS) (P0A800) | Escherichia coli | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 5 | F (L) | RNA polymerase sigma factor RpoD | polymer | 613 | 70352.2 | 1 | UniProt (Q0P6L9) Pfam (PF03979) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) UniProt (by SIFTS) (P00579) | Escherichia coli | Sigma-70 |
| 6 | G (P) | Nontemplate strand of T7A1 promoter DNA | polymer | 91 | 28137.1 | 1 | GenBank (312369) | Escherichia phage T7 | |
| 7 | H (Q) | Template strand of T7A1 promoter DNA | polymer | 91 | 27988.9 | 1 | GenBank (312369) | Escherichia phage T7 | |
| 8 | J, K, O (G, I, J) | CHAPSO | non-polymer | 631.9 | 3 | Chemie (1N7) | |||
| 9 | L (J) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 10 | M, N (J) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) |
Sequence modifications
J: 1 - 1407 (UniProt: A0A4S1NBU2)
P: 2 - 91 (GenBank: 312369)
Q: 1 - 90 (GenBank: 312369)
| PDB | External Database | Details |
|---|---|---|
| Val 1384 | Met 1384 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Dc 1 | - | insertion |
| PDB | External Database | Details |
|---|---|---|
| Dg 91 | - | insertion |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 552591.5 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 2050.8 | |
| All* | Total formula weight | 554642.3 |
