7MKJ
Cryo-EM structure of Escherichia coli RNA polymerase bound to T7A1 promoter DNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, I (G, H, R) | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 3 | UniProt (A0A073G207) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) UniProt (by SIFTS) (P0A7Z4) | Escherichia coli | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | C (I) | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V4) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) UniProt (by SIFTS) (P0A8V2) | Escherichia coli | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 3 | D (J) | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155366.8 | 1 | UniProt (A0A4S1NBU2) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P0A8T7) | Escherichia coli | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 4 | E (K) | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (P0A802) Pfam (PF01192) UniProt (by SIFTS) (P0A800) | Escherichia coli | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 5 | F (L) | RNA polymerase sigma factor RpoD | polymer | 613 | 70352.2 | 1 | UniProt (Q0P6L9) Pfam (PF03979) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) UniProt (by SIFTS) (P00579) | Escherichia coli | Sigma-70 |
| 6 | G (P) | Nontemplate strand of T7A1 promoter DNA | polymer | 91 | 28137.1 | 1 | GenBank (312369) | Escherichia phage T7 | |
| 7 | H (Q) | Template strand of T7A1 promoter DNA | polymer | 91 | 27988.9 | 1 | GenBank (312369) | Escherichia phage T7 | |
| 8 | J, K, O (G, I, J) | CHAPSO | non-polymer | 631.9 | 3 | Chemie (1N7) PubChem (16402373) PubChem (44119969) PubChem (44660322) PubChem (23636791) PubChem (23639399) PubChem (122146) PubChem (53393747) PubChem (50987332) PubChem (71464051) PubChem (3253739) PubChem (71305076) PubChem (90479263) PubChem (96545191) PubChem (124745618) PubChem (124901558) PubChem (124905421) PubChem (125027410) PubChem (10009199) PubChem (96327541) PubChem (96327542) PubChem (96327543) PubChem (96327544) PubChem (96545192) PubChem (102565431) PubChem (121392376) PubChem (122130148) PubChem (9895487) PubChem (144605089) PubChem (124901556) PubChem (124901561) PubChem (124901563) PubChem (124905422) PubChem (125027411) PubChem (129537355) PubChem (129537356) PubChem (145705472) PubChem (146030291) PubChem (146159852) | |||
| 9 | L (J) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) PubChem (888) | |||
| 10 | M, N (J) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) |
Sequence modifications
J: 1 - 1407 (UniProt: A0A4S1NBU2)
P: 2 - 91 (GenBank: 312369)
Q: 1 - 90 (GenBank: 312369)
| PDB | External Database | Details |
|---|---|---|
| Val 1384 | Met 1384 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Dc 1 | - | insertion |
| PDB | External Database | Details |
|---|---|---|
| Dg 91 | - | insertion |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 552591.5 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 2050.8 | |
| All* | Total formula weight | 554642.3 |
