7MKI
Cryo-EM structure of Escherichia coli RNA polymerase bound to lambda PR (-5G to C) promoter DNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (P) | Nontemplate strand of lambda PR DNA promoter (-5G to C) | polymer | 90 | 27841.8 | 1 | Escherichia virus Lambda | ||
| 2 | B, C (G, H) | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 2 | UniProt (A0A073G207) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) UniProt (by SIFTS) (P0A7Z4) | Escherichia coli | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 3 | D (I) | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V4) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) UniProt (by SIFTS) (P0A8V2) | Escherichia coli | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 4 | E (J) | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155366.8 | 1 | UniProt (A0A4S1NBU2) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P0A8T7) | Escherichia coli | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 5 | F (K) | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (P0A802) Pfam (PF01192) UniProt (by SIFTS) (P0A800) | Escherichia coli | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 6 | G (L) | RNA polymerase sigma factor RpoD | polymer | 613 | 70352.2 | 1 | UniProt (Q0P6L9) Pfam (PF03979) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) UniProt (by SIFTS) (P00579) | Escherichia coli | Sigma-70 |
| 7 | H (Q) | Template strand of lambda PR promoter DNA (-5C to G) | polymer | 90 | 27673.8 | 1 | Escherichia virus Lambda | ||
| 8 | I, J, N (I, J) | CHAPSO | non-polymer | 631.9 | 3 | Chemie (1N7) PubChem (122146) PubChem (3253739) PubChem (9895487) PubChem (16402373) PubChem (44119969) PubChem (44660322) PubChem (23636791) PubChem (23639399) PubChem (50987332) PubChem (71305076) PubChem (90479263) PubChem (96545191) PubChem (53393747) PubChem (124745618) PubChem (124901558) PubChem (124905421) PubChem (125027410) PubChem (10009199) PubChem (144605089) PubChem (71464051) PubChem (96327541) PubChem (96327542) PubChem (96327543) PubChem (96327544) PubChem (96545192) PubChem (102565431) PubChem (121392376) PubChem (122130148) PubChem (124901556) PubChem (124901561) PubChem (124901563) PubChem (124905422) PubChem (125027411) PubChem (129537355) PubChem (129537356) PubChem (145705472) PubChem (146030291) PubChem (146159852) | |||
| 9 | K (J) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) PubChem (888) | |||
| 10 | L, M (J) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) |
Sequence modifications
J: 1 - 1407 (UniProt: A0A4S1NBU2)
| PDB | External Database | Details |
|---|---|---|
| Val 1384 | Met 1384 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 515422.4 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 2050.8 | |
| All* | Total formula weight | 517473.1 |
