7MIS

Cryo-EM structure of SidJ-SdeC-CaM reaction intermediate complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Calmodulin-dependent glutamylase SidJ	polymer	756	87374.0	1	UniProt (Q5ZTK6)	Legionella pneumophila
2	B (B)	Calmodulin	polymer	150	16939.6	1	UniProt (P0DP24) Pfam (PF13499)	Homo sapiens (Human)
3	C (C)	SdeC	polymer	997	112775.3	1	UniProt (Q6RCQ8) Pfam (PF12252) Pfam (PF19048) UniProt (by SIFTS) (Q5ZTK8)	Legionella pneumophila
4	D (A)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	1	Chemie (ATP)
5	E, F (A)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	G (A)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
7	H (B)	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
8	I (C)	ADENOSINE MONOPHOSPHATE	non-polymer		347.2	1	Chemie (AMP)

Sequence modifications

A: 97 - 851 (UniProt: Q5ZTK6)

PDB	External Database	Details
Ser 96	-	expression tag

B: 1 - 149 (UniProt: P0DP24)

PDB	External Database	Details
Ser 0	-	expression tag

C: 231 - 1222 (UniProt: Q6RCQ8)

PDB	External Database	Details
Gly 226	-	expression tag
Ala 227	-	expression tag
Met 228	-	expression tag
Gly 229	-	expression tag
Ser 230	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	217089.0
Non-Polymers*	Number of molecules	6
	Total formula weight	966.1
All*	Total formula weight	218055.0

*Water molecules are not included.