7MIR
Cryo-EM structure of SidJ-SdeA-CaM reaction intermediate complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Calmodulin-dependent glutamylase SidJ | polymer | 756 | 87374.0 | 1 | UniProt (Q5ZTK6) | Legionella pneumophila | |
| 2 | B (B) | Calmodulin-2 | polymer | 150 | 16939.6 | 1 | UniProt (P0DP24) Pfam (PF13499) | Homo sapiens (Human) | |
| 3 | C (C) | Ubiquitinating/deubiquitinating enzyme SdeA | polymer | 965 | 109120.2 | 1 | UniProt (Q5ZTK4) Pfam (PF12252) Pfam (PF19048) | Legionella pneumophila | Effector protein SdeA |
| 4 | D (A) | ADENOSINE MONOPHOSPHATE | non-polymer | 347.2 | 1 | Chemie (AMP) | |||
| 5 | E, F (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 6 | G (A) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 1 | Chemie (ATP) | |||
| 7 | H (B) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) |
Sequence modifications
A: 97 - 851 (UniProt: Q5ZTK6)
B: 1 - 149 (UniProt: P0DP24)
C: 231 - 1190 (UniProt: Q5ZTK4)
| PDB | External Database | Details |
|---|---|---|
| Ser 96 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ser 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 226 | - | expression tag |
| Ala 227 | - | expression tag |
| Met 228 | - | expression tag |
| Gly 229 | - | expression tag |
| Ser 230 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 213433.9 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 943.1 | |
| All* | Total formula weight | 214377.0 |
