7MIR
Cryo-EM structure of SidJ-SdeA-CaM reaction intermediate complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Calmodulin-dependent glutamylase SidJ | polymer | 756 | 87374.0 | 1 | UniProt (Q5ZTK6) Pfam (PF26375) | Legionella pneumophila | |
| 2 | B (B) | Calmodulin-2 | polymer | 150 | 16939.6 | 1 | UniProt (P0DP24) Pfam (PF13499) | Homo sapiens (Human) | |
| 3 | C (C) | Ubiquitinating/deubiquitinating enzyme SdeA | polymer | 965 | 109120.2 | 1 | UniProt (Q5ZTK4) Pfam (PF12252) Pfam (PF19048) | Legionella pneumophila | Effector protein SdeA |
| 4 | D (A) | ADENOSINE MONOPHOSPHATE | non-polymer | 347.2 | 1 | Chemie (AMP) PubChem (16397049) PubChem (16760266) PubChem (224) PubChem (40467049) PubChem (40469468) PubChem (34768) PubChem (44348067) PubChem (83862) PubChem (45358029) PubChem (46874558) PubChem (40459109) PubChem (57358045) PubChem (57879893) PubChem (57879902) PubChem (46875232) PubChem (446048) PubChem (515384) PubChem (638042) PubChem (177678969) PubChem (6083) PubChem (1549371) PubChem (5316444) PubChem (92144436) PubChem (5742864) PubChem (122197642) PubChem (12358354) PubChem (12163610) PubChem (12358355) PubChem (162290139) PubChem (162946266) PubChem (171699505) | |||
| 5 | E, F (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) PubChem (888) | |||
| 6 | G (A) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 1 | Chemie (ATP) | |||
| 7 | H (B) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) PubChem (271) |
Sequence modifications
A: 97 - 851 (UniProt: Q5ZTK6)
B: 1 - 149 (UniProt: P0DP24)
C: 231 - 1190 (UniProt: Q5ZTK4)
| PDB | External Database | Details |
|---|---|---|
| Ser 96 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ser 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 226 | - | expression tag |
| Ala 227 | - | expression tag |
| Met 228 | - | expression tag |
| Gly 229 | - | expression tag |
| Ser 230 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 213433.9 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 943.1 | |
| All* | Total formula weight | 214377.0 |
