7MCS

Cryo-electron microscopy structure of TnsC(1-503)A225V bound to DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Transposon Tn7 transposition protein TnsC	polymer	523	59347.0	7	UniProt (P05846) Pfam (PF13401) In PDB	Escherichia coli	Protein E
2	H	DNA (5'-D(P*CP*GP*CP*GP*CP*GP*CP*GP*CP*GP*CP*GP*CP*GP*C)-3')	polymer	15	4572.9	1		synthetic construct
3	I	DNA (5'-D(P*GP*CP*GP*CP*GP*CP*GP*CP*GP*CP*GP*CP*GP*CP*G)-3')	polymer	15	4613.0	1		synthetic construct
4	B, C, D, E, F...	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	6	Chemie (ANP)
5	B, C, D, E, F...	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)

Sequence modifications

A, B, C, D, E, F, G: 1 - 503 (UniProt: P05846)

PDB	External Database	Details
Gly 2	Ser 2	engineered mutation
Val 225	Ala 225	engineered mutation
Pro 504	-	expression tag
Asn 505	-	expression tag
Ser 506	-	expression tag
Ser 507	-	expression tag
Ser 508	-	expression tag
Val 509	-	expression tag
Asp 510	-	expression tag
Lys 511	-	expression tag
Leu 512	-	expression tag
Ala 513	-	expression tag
Ala 514	-	expression tag
Ala 515	-	expression tag
Leu 516	-	expression tag
Glu 517	-	expression tag
His 518	-	expression tag
His 519	-	expression tag
His 520	-	expression tag
His 521	-	expression tag
His 522	-	expression tag
His 523	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	424614.8
Non-Polymers*	Number of molecules	12
	Total formula weight	3183.0
All*	Total formula weight	427797.8

*Water molecules are not included.