7M8E

E.coli RNAP-RapA elongation complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	DNA-directed RNA polymerase subunit alpha	polymer	329	36558.7	2	UniProt (A0A073G207) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) UniProt (by SIFTS) (P0A7Z4) In PDB	Escherichia coli	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	1	UniProt (P0A8V4) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) UniProt (by SIFTS) (P0A8V2) In PDB	Escherichia coli	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D	DNA-directed RNA polymerase subunit beta'	polymer	1416	156537.0	1	UniProt (D8ED86) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P0A8T7) In PDB	Escherichia coli	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E	DNA-directed RNA polymerase subunit omega	polymer	91	10249.5	1	UniProt (P0A802) Pfam (PF01192) UniProt (by SIFTS) (P0A800) In PDB	Escherichia coli	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	F	RNA polymerase-associated protein RapA	polymer	974	110726.8	1	UniProt (C3TR27) Pfam (PF18339) Pfam (PF18337) Pfam (PF00176) Pfam (PF00271) Pfam (PF12137) UniProt (by SIFTS) (P60240) In PDB	Escherichia coli	ATP-dependent helicase HepA
6	1	Nontemplate DNA (39-MER)	polymer	39	11949.7	1		Escherichia coli
7	2	Template DNA (39-MER)	polymer	39	12040.7	1		Escherichia coli
8	3	RNA (5'-R(P*AP*UP*CP*GP*GP*CP*UP*CP*A)-3')	polymer	9	2831.7	1		Escherichia coli
9	D	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
10	D	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

D: 1 - 1407 (UniProt: D8ED86)

PDB	External Database	Details
Leu 1408	-	expression tag
Glu 1409	-	expression tag
Val 1410	-	expression tag
His 1411	-	expression tag
His 1412	-	expression tag
His 1413	-	expression tag
His 1414	-	expression tag
His 1415	-	expression tag
His 1416	-	expression tag

F: 1 - 968 (UniProt: C3TR27)

PDB	External Database	Details
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	528273.7
Non-Polymers*	Number of molecules	3
	Total formula weight	155.1
All*	Total formula weight	528428.9

*Water molecules are not included.