7M85
Human DNA Pol eta S113A with dA-ended primer and dATP: in crystallo reaction for 80 s
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA polymerase eta | polymer | 435 | 48601.7 | 1 | UniProt (Q9Y253) Pfam (PF00817) Pfam (PF21704) Pfam (PF11799) | Homo sapiens (Human) | RAD30 homolog A,Xeroderma pigmentosum variant type protein |
| 2 | B (T) | DNA (5'-D(*CP*AP*TP*TP*TP*TP*GP*AP*CP*GP*CP*T)-3') | polymer | 12 | 3628.4 | 1 | Homo sapiens | ||
| 3 | C (P) | DNA (5'-D(*AP*GP*CP*GP*TP*CP*AP*A)-3') | polymer | 8 | 2435.6 | 1 | Homo sapiens | ||
| 4 | D (A) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 5 | E, H (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 6 | F (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 7 | G (A) | 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE | non-polymer | 491.2 | 1 | Chemie (DTP) | |||
| 8 | I, J, K (A, T, P) | water | water | 18.0 | 507 | Chemie (HOH) |
Sequence modifications
A: 1 - 432 (UniProt: Q9Y253)
| PDB | External Database | Details |
|---|---|---|
| Gly -2 | - | expression tag |
| Pro -1 | - | expression tag |
| His 0 | - | expression tag |
| Ala 113 | Ser 113 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 54665.7 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 672.0 | |
| All* | Total formula weight | 55337.7 |
