7M6U
Crystal structure of a circular permutation and computationally designed pro-enzyme of carboxypeptidase G2
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D | Carboxypeptidase G2 circular permuation pro-domain fusion | polymer | 446 | 47217.3 | 4 | UniProt (P06621) Pfam (PF01546) Pfam (PF07687) In PDB | Pseudomonas sp. (strain RS-16) | CPDG2,Folate hydrolase G2,Glutamate carboxypeptidase,Pteroylmonoglutamic acid hydrolase G2 |
2 | A, B, C, D | ZINC ION | non-polymer | 65.4 | 20 | Chemie (ZN) | |||
3 | A | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
4 | water | water | 18.0 | 132 | Chemie (HOH) |
Sequence modifications
A, B, C, D: -2 - 324 (UniProt: P06621)
A, B, C, D: 329 - 392 (UniProt: P06621)
A, B, C, D: 393 - 420 (PDB: 7M6U)
PDB | External Database | Details |
---|---|---|
Met -25 | - | initiating methionine |
Gly -24 | - | expression tag |
Ser -23 | - | expression tag |
Ser -22 | - | expression tag |
His -21 | - | expression tag |
His -20 | - | expression tag |
His -19 | - | expression tag |
His -18 | - | expression tag |
His -17 | - | expression tag |
His -16 | - | expression tag |
Ser -15 | - | expression tag |
Ser -14 | - | expression tag |
Gly -13 | - | expression tag |
Leu -12 | - | expression tag |
Val -11 | - | expression tag |
Pro -10 | - | expression tag |
Arg -9 | - | expression tag |
Gly -8 | - | expression tag |
Ser -7 | - | expression tag |
His -6 | - | expression tag |
Met -5 | - | expression tag |
Gly -4 | - | expression tag |
Ser -3 | - | expression tag |
Ala 86 | Lys 177 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Gly 325 | - | linker |
Thr 326 | - | linker |
Ala 327 | - | linker |
Ser 328 | - | linker |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 188869.1 | |
Non-Polymers* | Number of molecules | 21 |
Total formula weight | 1404.2 | |
All* | Total formula weight | 190273.4 |