7M6J

Human Septin Hexameric Complex SEPT2G/SEPT6/SEPT7 by Single Particle Cryo-EM

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, F (A, F)	Septin-2	polymer	275	31748.4	2	UniProt (Q15019) Pfam (PF00735)	Homo sapiens (Human)	Neural precursor cell expressed developmentally down-regulated protein 5,NEDD-5
2	B, E (B, E)	Septin-6	polymer	427	48948.7	2	UniProt (Q14141) Pfam (PF00735)	Homo sapiens (Human)
3	C, D (C, D)	Septin-7	polymer	432	50456.6	2	UniProt (Q16181) Pfam (PF00735)	Homo sapiens (Human)	CDC10 protein homolog
4	G, I, J, M (A, C, D, F)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	4	Chemie (GDP)
5	H, L (B, E)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)
6	K (E)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

A, F: 35 - 308 (UniProt: Q15019)

PDB	External Database	Details
Met 34	-	initiating methionine

C, D: 20 - 437 (UniProt: Q16181)

PDB	External Database	Details
Met 6	-	initiating methionine
Gly 7	-	expression tag
Ser 8	-	expression tag
Ser 9	-	expression tag
His 10	-	expression tag
His 11	-	expression tag
His 12	-	expression tag
His 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
Ser 16	-	expression tag
Gln 17	-	expression tag
Asp 18	-	expression tag
Pro 19	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	262307.4
Non-Polymers*	Number of molecules	7
	Total formula weight	2843.5
All*	Total formula weight	265150.9

*Water molecules are not included.