7M5D
Cryo-EM structure of a non-rotated E.coli 70S ribosome in complex with RF3-GTP, RF1 and P-tRNA (state I)
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (1) | 23S ribosomal RNA | polymer | 2903 | 941526.4 | 1 | Escherichia coli | ||
| 2 | B (2) | 16S ribosomal RNA | polymer | 1534 | 497406.0 | 1 | GenBank (1758835854) | Escherichia coli | |
| 3 | C (3) | 5S ribosomal RNA | polymer | 120 | 38790.1 | 1 | GenBank (1273279017) | Escherichia coli | |
| 4 | D (4) | mRNA | polymer | 15 | 4729.9 | 1 | Escherichia coli | ||
| 5 | E (5) | P-tRNA | polymer | 76 | 24565.8 | 1 | Escherichia coli | ||
| 6 | F (B) | 50S ribosomal protein L2 | polymer | 271 | 29663.2 | 1 | UniProt (P60422) Pfam (PF00181) Pfam (PF03947) | Escherichia coli | Large ribosomal subunit protein uL2 |
| 7 | G (C) | 50S ribosomal protein L3 | polymer | 209 | 22277.5 | 1 | UniProt (P60438) Pfam (PF00297) | Escherichia coli | Large ribosomal subunit protein uL3 |
| 8 | H (D) | 50S ribosomal protein L4 | polymer | 201 | 22121.6 | 1 | UniProt (D7Z9F6) Pfam (PF00573) UniProt (by SIFTS) (P60723) | Escherichia coli | |
| 9 | I (E) | 50S ribosomal protein L5 | polymer | 177 | 20073.2 | 1 | UniProt (H4IXW7) Pfam (PF00281) Pfam (PF00673) UniProt (by SIFTS) (P62399) | Escherichia coli | |
| 10 | J (F) | 50S ribosomal protein L6 | polymer | 175 | 18672.4 | 1 | UniProt (A0A5B7MHD1) Pfam (PF00347) UniProt (by SIFTS) (P0AG55) | Escherichia coli | |
| 11 | K (G) | 50S ribosomal protein L9 | polymer | 149 | 15789.0 | 1 | UniProt (D7ZI15) Pfam (PF01281) Pfam (PF03948) UniProt (by SIFTS) (P0A7R1) | Escherichia coli | |
| 12 | L (J) | 50S ribosomal protein L13 | polymer | 142 | 16050.6 | 1 | UniProt (D7ZET0) Pfam (PF00572) UniProt (by SIFTS) (P0AA10) | Escherichia coli | |
| 13 | M (K) | 50S ribosomal protein L14 | polymer | 123 | 13565.1 | 1 | UniProt (D7Z9G5) Pfam (PF00238) UniProt (by SIFTS) (P0ADY3) | Escherichia coli | |
| 14 | N (L) | 50S ribosomal protein L15 | polymer | 144 | 15008.5 | 1 | UniProt (A0A037Y8L6) Pfam (PF00828) UniProt (by SIFTS) (P02413) | Escherichia coli | |
| 15 | O (M) | 50S ribosomal protein L16 | polymer | 136 | 15312.3 | 1 | UniProt (P0ADY7) Pfam (PF00252) | Escherichia coli | Large ribosomal subunit protein uL16 |
| 16 | P (N) | 50S ribosomal protein L17 | polymer | 119 | 13592.8 | 1 | UniProt (A0A2T1LEK9) Pfam (PF01196) UniProt (by SIFTS) (P0AG44) | Escherichia coli | |
| 17 | Q (O) | 50S ribosomal protein L18 | polymer | 116 | 12663.5 | 1 | UniProt (F4SQ31) Pfam (PF00861) UniProt (by SIFTS) (P0C018) | Escherichia coli | |
| 18 | R (P) | 50S ribosomal protein L19 | polymer | 114 | 13028.1 | 1 | UniProt (I2XAW2) Pfam (PF01245) UniProt (by SIFTS) (P0A7K6) | Escherichia coli | |
| 19 | S (Q) | 50S ribosomal protein L20 | polymer | 117 | 13396.8 | 1 | UniProt (A0A7B6WTL0) Pfam (PF00453) UniProt (by SIFTS) (P0A7L3) | Escherichia coli | |
| 20 | T (R) | 50S ribosomal protein L21 | polymer | 103 | 11586.4 | 1 | UniProt (D7ZEN7) Pfam (PF00829) UniProt (by SIFTS) (P0AG48) | Escherichia coli | |
| 21 | U (S) | 50S ribosomal protein L22 | polymer | 110 | 12253.4 | 1 | UniProt (D7Z9G0) Pfam (PF00237) UniProt (by SIFTS) (P61175) | Escherichia coli | |
| 22 | V (T) | 50S ribosomal protein L23 | polymer | 94 | 10661.6 | 1 | UniProt (S1EUY1) Pfam (PF00276) UniProt (by SIFTS) (P0ADZ0) | Escherichia coli | |
| 23 | W (U) | 50S ribosomal protein L24 | polymer | 103 | 11208.1 | 1 | UniProt (F4SQ36) Pfam (PF00467) Pfam (PF17136) UniProt (by SIFTS) (P60624) | Escherichia coli | |
| 24 | X (V) | 50S ribosomal protein L25 | polymer | 94 | 10713.5 | 1 | UniProt (D7XH79) Pfam (PF01386) UniProt (by SIFTS) (P68919) | Escherichia coli | |
| 25 | Y (W) | 50S ribosomal protein L27 | polymer | 76 | 8275.5 | 1 | UniProt (A0A073FRE3) Pfam (PF01016) UniProt (by SIFTS) (P0A7L8) | Escherichia coli | |
| 26 | Z (X) | 50S ribosomal protein L28 | polymer | 77 | 8896.4 | 1 | UniProt (A0A5B7P765) Pfam (PF00830) UniProt (by SIFTS) (P0A7M2) | Escherichia coli | |
| 27 | AA (Y) | 50S ribosomal protein L29 | polymer | 62 | 7155.3 | 1 | UniProt (I2X052) Pfam (PF00831) UniProt (by SIFTS) (P0A7M6) | Escherichia coli | |
| 28 | BA (Z) | 50S ribosomal protein L30 | polymer | 58 | 6423.6 | 1 | UniProt (A0A1X3K2S4) Pfam (PF00327) UniProt (by SIFTS) (P0AG51) | Escherichia coli | |
| 29 | CA (a) | 50S ribosomal protein L31 | polymer | 66 | 7516.7 | 1 | UniProt (A0A770S7V1) Pfam (PF01197) UniProt (by SIFTS) (P0A7M9) | Escherichia coli | |
| 30 | DA (b) | 50S ribosomal protein L32 | polymer | 56 | 6332.2 | 1 | UniProt (H4J6G2) Pfam (PF01783) UniProt (by SIFTS) (P0A7N4) | Escherichia coli | |
| 31 | EA (c) | 50S ribosomal protein L33 | polymer | 52 | 6057.2 | 1 | UniProt (T9DEE3) Pfam (PF00471) UniProt (by SIFTS) (P0A7N9) | Escherichia coli | |
| 32 | FA (d) | 50S ribosomal protein L34 | polymer | 46 | 5397.5 | 1 | UniProt (B7MGC4) Pfam (PF00468) UniProt (by SIFTS) (P0A7P5) | Escherichia coli | |
| 33 | GA (e) | 50S ribosomal protein L35 | polymer | 64 | 7181.8 | 1 | UniProt (A0A7G1MI33) Pfam (PF01632) UniProt (by SIFTS) (P0A7Q1) | Escherichia coli | |
| 34 | HA (f) | 50S ribosomal protein L36 | polymer | 38 | 4377.4 | 1 | UniProt (A0A0E2L017) Pfam (PF00444) UniProt (by SIFTS) (P0A7Q6) | Escherichia coli | |
| 35 | IA (g) | 30S ribosomal protein S2 | polymer | 225 | 25072.9 | 1 | UniProt (C3TPN2) Pfam (PF00318) UniProt (by SIFTS) (P0A7V0) | Escherichia coli | |
| 36 | JA (h) | 30S ribosomal protein S3 | polymer | 208 | 23249.0 | 1 | UniProt (A0A376HTV6) Pfam (PF07650) Pfam (PF00189) UniProt (by SIFTS) (P0A7V3) | Escherichia coli | |
| 37 | KA (i) | 30S ribosomal protein S4 | polymer | 205 | 23383.0 | 1 | UniProt (L3PZ69) Pfam (PF00163) Pfam (PF01479) UniProt (by SIFTS) (P0A7V8) | Escherichia coli | |
| 38 | LA (j) | 30S ribosomal protein S5 | polymer | 156 | 16475.0 | 1 | UniProt (T9SGC3) Pfam (PF00333) Pfam (PF03719) UniProt (by SIFTS) (P0A7W1) | Escherichia coli | |
| 39 | MA (k) | 30S ribosomal protein S6 | polymer | 104 | 12126.0 | 1 | UniProt (A0A376ZL25) Pfam (PF01250) UniProt (by SIFTS) (P02358) | Escherichia coli | |
| 40 | NA (l) | 30S ribosomal protein S7 | polymer | 151 | 16861.5 | 1 | UniProt (S1D5F0) Pfam (PF00177) UniProt (by SIFTS) (P02359) | Escherichia coli | |
| 41 | OA (m) | 30S ribosomal protein S8 | polymer | 129 | 14015.4 | 1 | UniProt (D8A1L7) Pfam (PF00410) UniProt (by SIFTS) (P0A7W7) | Escherichia coli | |
| 42 | PA (n) | 30S ribosomal protein S9 | polymer | 127 | 14554.9 | 1 | UniProt (T9TH92) Pfam (PF00380) UniProt (by SIFTS) (P0A7X3) | Escherichia coli | |
| 43 | QA (o) | 30S ribosomal protein S10 | polymer | 99 | 11254.0 | 1 | UniProt (A0A073G203) Pfam (PF00338) UniProt (by SIFTS) (P0A7R5) | Escherichia coli | |
| 44 | RA (p) | 30S ribosomal protein S11 | polymer | 117 | 12487.2 | 1 | UniProt (P0A7R9) Pfam (PF00411) | Escherichia coli | Small ribosomal subunit protein uS11 |
| 45 | SA (q) | 30S ribosomal protein S12 | polymer | 123 | 13683.1 | 1 | UniProt (I2UHF1) Pfam (PF00164) UniProt (by SIFTS) (P0A7S3) | Escherichia coli | |
| 46 | TA (r) | 30S ribosomal protein S13 | polymer | 116 | 12868.1 | 1 | UniProt (I2X719) Pfam (PF00416) UniProt (by SIFTS) (P0A7S9) | Escherichia coli | |
| 47 | UA (s) | 30S ribosomal protein S14 | polymer | 100 | 11475.4 | 1 | UniProt (A0A1X3K3H0) Pfam (PF00253) UniProt (by SIFTS) (P0AG59) | Escherichia coli | |
| 48 | VA (t) | 30S ribosomal protein S15 | polymer | 88 | 10159.6 | 1 | UniProt (D7XN21) Pfam (PF00312) UniProt (by SIFTS) (P0ADZ4) | Escherichia coli | |
| 49 | WA (u) | 30S ribosomal protein S16 | polymer | 82 | 9207.6 | 1 | UniProt (B7MIU7) Pfam (PF00886) UniProt (by SIFTS) (P0A7T3) | Escherichia coli | |
| 50 | XA (v) | 30S ribosomal protein S17 | polymer | 80 | 9263.9 | 1 | UniProt (A0A080IK26) Pfam (PF00366) UniProt (by SIFTS) (P0AG63) | Escherichia coli | |
| 51 | YA (w) | 30S ribosomal protein S18 | polymer | 66 | 7734.9 | 1 | UniProt (I2SQR9) Pfam (PF01084) UniProt (by SIFTS) (P0A7T7) | Escherichia coli | |
| 52 | ZA (x) | 30S ribosomal protein S19 | polymer | 83 | 9421.0 | 1 | UniProt (A0A7G6HV07) Pfam (PF00203) UniProt (by SIFTS) (P0A7U3) | Escherichia coli | |
| 53 | AB (y) | 30S ribosomal protein S20 | polymer | 86 | 9577.3 | 1 | UniProt (I4T5W9) Pfam (PF01649) UniProt (by SIFTS) (P0A7U7) | Escherichia coli | |
| 54 | BB (z) | 30S ribosomal protein S21 | polymer | 70 | 8392.8 | 1 | UniProt (E9TH65) Pfam (PF01165) UniProt (by SIFTS) (P68679) | Escherichia coli | |
| 55 | CB (7) | Peptide chain release factor 3 | polymer | 525 | 58891.1 | 1 | UniProt (B7LEM0) Pfam (PF00009) Pfam (PF03144) Pfam (PF16658) UniProt (by SIFTS) (P0A7I4) | Escherichia coli | RF-3 |
| 56 | DB (I) | 50S ribosomal protein L11 | polymer | 141 | 14763.2 | 1 | UniProt (I2WL52) Pfam (PF03946) Pfam (PF00298) UniProt (by SIFTS) (P0A7J7) | Escherichia coli | |
| 57 | EB (H) | 50S ribosomal protein L10 | polymer | 131 | 14112.3 | 1 | UniProt (A0A780WW86) Pfam (PF00466) UniProt (by SIFTS) (P0A7J3) | Escherichia coli | |
| 58 | FB (A) | Peptide chain release factor 1 | polymer | 360 | 40579.3 | 1 | UniProt (A0A073V365) Pfam (PF03462) Pfam (PF00472) UniProt (by SIFTS) (P0A7I0) | Escherichia coli | RF-1 |
| 59 | GB (6) | FME-PHE-PHE | polymer | 3 | 471.6 | 1 | Escherichia coli | ||
| 60 | HB (a) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) |
Sequence modifications
7: 3 - 527 (UniProt: B7LEM0)
A: 1 - 360 (UniProt: A0A073V365)
| PDB | External Database | Details |
|---|---|---|
| Gly 151 | Pro 151 | conflict |
| Gly 295 | Lys 295 | conflict |
| Gly 296 | Phe 296 | conflict |
| Gly 297 | Thr 297 | conflict |
| Lys 477 | Ala 477 | conflict |
| Ala 491 | Ser 491 | conflict |
| Ala 492 | Gln 492 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ser 3 | Pro 3 | conflict |
| Cys 167 | Ser 167 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 59 |
| Total formula weight | 2272349.2 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 65.4 | |
| All* | Total formula weight | 2272414.6 |
