7M5D
Cryo-EM structure of a non-rotated E.coli 70S ribosome in complex with RF3-GTP, RF1 and P-tRNA (state I)
This is a non-PDB format compatible entry.
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | 1 | 23S ribosomal RNA | polymer | 2903 | 941526.4 | 1 | Escherichia coli | ||
2 | 2 | 16S ribosomal RNA | polymer | 1534 | 497406.0 | 1 | GenBank (1758835854) | Escherichia coli | |
3 | 3 | 5S ribosomal RNA | polymer | 120 | 38790.1 | 1 | GenBank (1273279017) | Escherichia coli | |
4 | 4 | mRNA | polymer | 15 | 4729.9 | 1 | Escherichia coli | ||
5 | 5 | P-tRNA | polymer | 76 | 24565.8 | 1 | Escherichia coli | ||
6 | B | 50S ribosomal protein L2 | polymer | 271 | 29663.2 | 1 | UniProt (P60422) Pfam (PF00181) Pfam (PF03947) In PDB | Escherichia coli | Large ribosomal subunit protein uL2 |
7 | C | 50S ribosomal protein L3 | polymer | 209 | 22277.5 | 1 | UniProt (P60438) Pfam (PF00297) In PDB | Escherichia coli | Large ribosomal subunit protein uL3 |
8 | D | 50S ribosomal protein L4 | polymer | 201 | 22121.6 | 1 | UniProt (D7Z9F6) UniProt (by SIFTS) (P60723) Pfam (PF00573) In PDB | Escherichia coli | |
9 | E | 50S ribosomal protein L5 | polymer | 177 | 20073.2 | 1 | UniProt (H4IXW7) UniProt (by SIFTS) (P62399) Pfam (PF00281) Pfam (PF00673) In PDB | Escherichia coli | |
10 | F | 50S ribosomal protein L6 | polymer | 175 | 18672.4 | 1 | UniProt (A0A5B7MHD1) Pfam (PF00347) UniProt (by SIFTS) (P0AG55) In PDB | Escherichia coli | |
11 | G | 50S ribosomal protein L9 | polymer | 149 | 15789.0 | 1 | UniProt (D7ZI15) UniProt (by SIFTS) (P0A7R1) Pfam (PF01281) Pfam (PF03948) In PDB | Escherichia coli | |
12 | J | 50S ribosomal protein L13 | polymer | 142 | 16050.6 | 1 | UniProt (D7ZET0) UniProt (by SIFTS) (P0AA10) Pfam (PF00572) In PDB | Escherichia coli | |
13 | K | 50S ribosomal protein L14 | polymer | 123 | 13565.1 | 1 | UniProt (D7Z9G5) UniProt (by SIFTS) (P0ADY3) Pfam (PF00238) In PDB | Escherichia coli | |
14 | L | 50S ribosomal protein L15 | polymer | 144 | 15008.5 | 1 | UniProt (A0A037Y8L6) UniProt (by SIFTS) (P02413) Pfam (PF00828) In PDB | Escherichia coli | |
15 | M | 50S ribosomal protein L16 | polymer | 136 | 15312.3 | 1 | UniProt (P0ADY7) Pfam (PF00252) In PDB | Escherichia coli | Large ribosomal subunit protein uL16 |
16 | N | 50S ribosomal protein L17 | polymer | 119 | 13592.8 | 1 | UniProt (A0A2T1LEK9) UniProt (by SIFTS) (P0AG44) Pfam (PF01196) In PDB | Escherichia coli | |
17 | O | 50S ribosomal protein L18 | polymer | 116 | 12663.5 | 1 | UniProt (F4SQ31) UniProt (by SIFTS) (P0C018) Pfam (PF00861) In PDB | Escherichia coli | |
18 | P | 50S ribosomal protein L19 | polymer | 114 | 13028.1 | 1 | UniProt (I2XAW2) UniProt (by SIFTS) (P0A7K6) Pfam (PF01245) In PDB | Escherichia coli | |
19 | Q | 50S ribosomal protein L20 | polymer | 117 | 13396.8 | 1 | UniProt (A0A7B6WTL0) UniProt (by SIFTS) (P0A7L3) Pfam (PF00453) In PDB | Escherichia coli | |
20 | R | 50S ribosomal protein L21 | polymer | 103 | 11586.4 | 1 | UniProt (D7ZEN7) UniProt (by SIFTS) (P0AG48) Pfam (PF00829) In PDB | Escherichia coli | |
21 | S | 50S ribosomal protein L22 | polymer | 110 | 12253.4 | 1 | UniProt (D7Z9G0) UniProt (by SIFTS) (P61175) Pfam (PF00237) In PDB | Escherichia coli | |
22 | T | 50S ribosomal protein L23 | polymer | 94 | 10661.6 | 1 | UniProt (S1EUY1) UniProt (by SIFTS) (P0ADZ0) Pfam (PF00276) In PDB | Escherichia coli | |
23 | U | 50S ribosomal protein L24 | polymer | 103 | 11208.1 | 1 | UniProt (F4SQ36) UniProt (by SIFTS) (P60624) Pfam (PF00467) Pfam (PF17136) In PDB | Escherichia coli | |
24 | V | 50S ribosomal protein L25 | polymer | 94 | 10713.5 | 1 | UniProt (D7XH79) UniProt (by SIFTS) (P68919) Pfam (PF01386) In PDB | Escherichia coli | |
25 | W | 50S ribosomal protein L27 | polymer | 76 | 8275.5 | 1 | UniProt (A0A073FRE3) UniProt (by SIFTS) (P0A7L8) Pfam (PF01016) In PDB | Escherichia coli | |
26 | X | 50S ribosomal protein L28 | polymer | 77 | 8896.4 | 1 | UniProt (A0A5B7P765) UniProt (by SIFTS) (P0A7M2) Pfam (PF00830) In PDB | Escherichia coli | |
27 | Y | 50S ribosomal protein L29 | polymer | 62 | 7155.3 | 1 | UniProt (I2X052) UniProt (by SIFTS) (P0A7M6) Pfam (PF00831) In PDB | Escherichia coli | |
28 | Z | 50S ribosomal protein L30 | polymer | 58 | 6423.6 | 1 | UniProt (A0A1X3K2S4) UniProt (by SIFTS) (P0AG51) Pfam (PF00327) In PDB | Escherichia coli | |
29 | a | 50S ribosomal protein L31 | polymer | 66 | 7516.7 | 1 | UniProt (A0A770S7V1) UniProt (by SIFTS) (P0A7M9) Pfam (PF01197) In PDB | Escherichia coli | |
30 | b | 50S ribosomal protein L32 | polymer | 56 | 6332.2 | 1 | UniProt (H4J6G2) UniProt (by SIFTS) (P0A7N4) Pfam (PF01783) In PDB | Escherichia coli | |
31 | c | 50S ribosomal protein L33 | polymer | 52 | 6057.2 | 1 | UniProt (T9DEE3) UniProt (by SIFTS) (P0A7N9) Pfam (PF00471) In PDB | Escherichia coli | |
32 | d | 50S ribosomal protein L34 | polymer | 46 | 5397.5 | 1 | UniProt (B7MGC4) UniProt (by SIFTS) (P0A7P5) Pfam (PF00468) In PDB | Escherichia coli | |
33 | e | 50S ribosomal protein L35 | polymer | 64 | 7181.8 | 1 | UniProt (A0A7G1MI33) UniProt (by SIFTS) (P0A7Q1) Pfam (PF01632) In PDB | Escherichia coli | |
34 | f | 50S ribosomal protein L36 | polymer | 38 | 4377.4 | 1 | UniProt (A0A0E2L017) UniProt (by SIFTS) (P0A7Q6) Pfam (PF00444) In PDB | Escherichia coli | |
35 | g | 30S ribosomal protein S2 | polymer | 225 | 25072.9 | 1 | UniProt (C3TPN2) UniProt (by SIFTS) (P0A7V0) Pfam (PF00318) In PDB | Escherichia coli | |
36 | h | 30S ribosomal protein S3 | polymer | 208 | 23249.0 | 1 | UniProt (A0A376HTV6) UniProt (by SIFTS) (P0A7V3) Pfam (PF07650) Pfam (PF00189) In PDB | Escherichia coli | |
37 | i | 30S ribosomal protein S4 | polymer | 205 | 23383.0 | 1 | UniProt (L3PZ69) UniProt (by SIFTS) (P0A7V8) Pfam (PF00163) Pfam (PF01479) In PDB | Escherichia coli | |
38 | j | 30S ribosomal protein S5 | polymer | 156 | 16475.0 | 1 | UniProt (T9SGC3) UniProt (by SIFTS) (P0A7W1) Pfam (PF00333) Pfam (PF03719) In PDB | Escherichia coli | |
39 | k | 30S ribosomal protein S6 | polymer | 104 | 12126.0 | 1 | UniProt (A0A376ZL25) UniProt (by SIFTS) (P02358) Pfam (PF01250) In PDB | Escherichia coli | |
40 | l | 30S ribosomal protein S7 | polymer | 151 | 16861.5 | 1 | UniProt (S1D5F0) UniProt (by SIFTS) (P02359) Pfam (PF00177) In PDB | Escherichia coli | |
41 | m | 30S ribosomal protein S8 | polymer | 129 | 14015.4 | 1 | UniProt (D8A1L7) UniProt (by SIFTS) (P0A7W7) Pfam (PF00410) In PDB | Escherichia coli | |
42 | n | 30S ribosomal protein S9 | polymer | 127 | 14554.9 | 1 | UniProt (T9TH92) UniProt (by SIFTS) (P0A7X3) Pfam (PF00380) In PDB | Escherichia coli | |
43 | o | 30S ribosomal protein S10 | polymer | 99 | 11254.0 | 1 | UniProt (A0A073G203) UniProt (by SIFTS) (P0A7R5) Pfam (PF00338) In PDB | Escherichia coli | |
44 | p | 30S ribosomal protein S11 | polymer | 117 | 12487.2 | 1 | UniProt (P0A7R9) Pfam (PF00411) In PDB | Escherichia coli | Small ribosomal subunit protein uS11 |
45 | q | 30S ribosomal protein S12 | polymer | 123 | 13683.1 | 1 | UniProt (I2UHF1) UniProt (by SIFTS) (P0A7S3) Pfam (PF00164) In PDB | Escherichia coli | |
46 | r | 30S ribosomal protein S13 | polymer | 116 | 12868.1 | 1 | UniProt (I2X719) UniProt (by SIFTS) (P0A7S9) Pfam (PF00416) In PDB | Escherichia coli | |
47 | s | 30S ribosomal protein S14 | polymer | 100 | 11475.4 | 1 | UniProt (A0A1X3K3H0) UniProt (by SIFTS) (P0AG59) Pfam (PF00253) In PDB | Escherichia coli | |
48 | t | 30S ribosomal protein S15 | polymer | 88 | 10159.6 | 1 | UniProt (D7XN21) UniProt (by SIFTS) (P0ADZ4) Pfam (PF00312) In PDB | Escherichia coli | |
49 | u | 30S ribosomal protein S16 | polymer | 82 | 9207.6 | 1 | UniProt (B7MIU7) UniProt (by SIFTS) (P0A7T3) Pfam (PF00886) In PDB | Escherichia coli | |
50 | v | 30S ribosomal protein S17 | polymer | 80 | 9263.9 | 1 | UniProt (A0A080IK26) UniProt (by SIFTS) (P0AG63) Pfam (PF00366) In PDB | Escherichia coli | |
51 | w | 30S ribosomal protein S18 | polymer | 66 | 7734.9 | 1 | UniProt (I2SQR9) UniProt (by SIFTS) (P0A7T7) Pfam (PF01084) In PDB | Escherichia coli | |
52 | x | 30S ribosomal protein S19 | polymer | 83 | 9421.0 | 1 | UniProt (A0A7G6HV07) UniProt (by SIFTS) (P0A7U3) Pfam (PF00203) In PDB | Escherichia coli | |
53 | y | 30S ribosomal protein S20 | polymer | 86 | 9577.3 | 1 | UniProt (I4T5W9) UniProt (by SIFTS) (P0A7U7) Pfam (PF01649) In PDB | Escherichia coli | |
54 | z | 30S ribosomal protein S21 | polymer | 70 | 8392.8 | 1 | UniProt (E9TH65) UniProt (by SIFTS) (P68679) Pfam (PF01165) In PDB | Escherichia coli | |
55 | 7 | Peptide chain release factor 3 | polymer | 525 | 58891.1 | 1 | UniProt (B7LEM0) Pfam (PF00009) Pfam (PF03144) Pfam (PF16658) UniProt (by SIFTS) (P0A7I4) In PDB | Escherichia coli | RF-3 |
56 | I | 50S ribosomal protein L11 | polymer | 141 | 14763.2 | 1 | UniProt (I2WL52) UniProt (by SIFTS) (P0A7J7) Pfam (PF03946) Pfam (PF00298) In PDB | Escherichia coli | |
57 | H | 50S ribosomal protein L10 | polymer | 131 | 14112.3 | 1 | UniProt (A0A780WW86) UniProt (by SIFTS) (P0A7J3) Pfam (PF00466) In PDB | Escherichia coli | |
58 | A | Peptide chain release factor 1 | polymer | 360 | 40579.3 | 1 | UniProt (A0A073V365) UniProt (by SIFTS) (P0A7I0) Pfam (PF03462) Pfam (PF00472) In PDB | Escherichia coli | RF-1 |
59 | 6 | FME-PHE-PHE | polymer | 3 | 471.6 | 1 | Escherichia coli | ||
60 | a | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) |
Sequence modifications
7: 3 - 527 (UniProt: B7LEM0)
A: 1 - 360 (UniProt: A0A073V365)
PDB | External Database | Details |
---|---|---|
Gly 151 | Pro 151 | conflict |
Gly 295 | Lys 295 | conflict |
Gly 296 | Phe 296 | conflict |
Gly 297 | Thr 297 | conflict |
Lys 477 | Ala 477 | conflict |
Ala 491 | Ser 491 | conflict |
Ala 492 | Gln 492 | conflict |
PDB | External Database | Details |
---|---|---|
Ser 3 | Pro 3 | conflict |
Cys 167 | Ser 167 | conflict |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 59 |
Total formula weight | 2272349.2 | |
Non-Polymers* | Number of molecules | 1 |
Total formula weight | 65.4 | |
All* | Total formula weight | 2272414.6 |