7M3Q
Structure of the Smurf2 HECT Domain with a High Affinity Ubiquitin Variant (UbV)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | E3 ubiquitin-protein ligase SMURF2 | polymer | 387 | 45254.8 | 1 | UniProt (Q9HAU4) Pfam (PF00632) | Homo sapiens (Human) | hSMURF2,HECT-type E3 ubiquitin transferase SMURF2,SMAD ubiquitination regulatory factor 2,SMAD-specific E3 ubiquitin-protein ligase 2 |
| 2 | B (B) | Ubiquitin variant | polymer | 98 | 11519.2 | 1 | UniProt (P62987) Pfam (PF00240) | Homo sapiens (Human) | |
| 3 | C, D (A) | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
| 4 | E, F (A) | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) | |||
| 5 | G, H, I, J, K... (A) | CHLORIDE ION | non-polymer | 35.5 | 6 | Chemie (CL) | |||
| 6 | M (A) | 2,3-DIHYDROXY-1,4-DITHIOBUTANE | non-polymer | 154.3 | 1 | Chemie (DTT) | |||
| 7 | N (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 1 | Chemie (EDO) | |||
| 8 | O (A) | water | water | 18.0 | 54 | Chemie (HOH) |
Sequence modifications
A: 366 - 748 (UniProt: Q9HAU4)
B: 2 - 74 (UniProt: P62987)
| PDB | External Database | Details |
|---|---|---|
| Gly 362 | - | expression tag |
| Ala 363 | - | expression tag |
| Met 364 | - | expression tag |
| Ala 365 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met -23 | - | expression tag |
| Ala -22 | - | expression tag |
| His -21 | - | expression tag |
| His -20 | - | expression tag |
| His -19 | - | expression tag |
| His -18 | - | expression tag |
| His -17 | - | expression tag |
| His -16 | - | expression tag |
| Val -15 | - | expression tag |
| Thr -14 | - | expression tag |
| Ser -13 | - | expression tag |
| Leu -12 | - | expression tag |
| Tyr -11 | - | expression tag |
| Lys -10 | - | expression tag |
| Lys -9 | - | expression tag |
| Ala -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Asp -6 | - | expression tag |
| Tyr -5 | - | expression tag |
| Lys -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Lys 0 | - | expression tag |
| Met 1 | - | initiating methionine |
| Leu 2 | Gln 2 | variant |
| Trp 6 | Lys 6 | variant |
| Phe 8 | Leu 8 | variant |
| Lys 9 | Thr 9 | variant |
| Asn 11 | Lys 11 | variant |
| Arg 47 | Gly 47 | variant |
| Leu 62 | Gln 62 | variant |
| Leu 68 | His 68 | variant |
| Arg 71 | Leu 71 | variant |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 56774.0 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 667.1 | |
| All* | Total formula weight | 57441.1 |
