7M0A
Incomplete in crystallo incorporation by DNA Polymerase Lambda bound to blunt-ended DSB substrate and incoming dTTP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA polymerase lambda | polymer | 346 | 38551.0 | 1 | UniProt (Q9UGP5) Pfam (PF14716) Pfam (PF10391) Pfam (PF14792) Pfam (PF14791) | Homo sapiens (Human) | Pol Lambda,DNA polymerase beta-2,Pol beta2,DNA polymerase kappa |
| 2 | B (T) | DNA (5'-D(*CP*GP*GP*CP*A)-3') | polymer | 5 | 1505.0 | 1 | synthetic construct | ||
| 3 | C (P) | DNA (5'-D(*CP*AP*GP*TP*GP*CP*T)-3') | polymer | 7 | 2113.4 | 1 | synthetic construct | ||
| 4 | D (D) | DNA (5'-D(P*GP*CP*CP*G)-3') | polymer | 4 | 1191.8 | 1 | synthetic construct | ||
| 5 | E (U) | DNA (5'-D(*GP*CP*AP*CP*TP*G)-3') | polymer | 6 | 1809.2 | 1 | synthetic construct | ||
| 6 | F (A) | THYMIDINE-5'-TRIPHOSPHATE | non-polymer | 482.2 | 1 | Chemie (TTP) | |||
| 7 | G (A) | PYROPHOSPHATE | non-polymer | 178.0 | 1 | Chemie (PPV) | |||
| 8 | H, J (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 9 | I (A) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 10 | K, L, M (A) | SODIUM ION | non-polymer | 23.0 | 3 | Chemie (NA) | |||
| 11 | N, O, P (A) | CHLORIDE ION | non-polymer | 35.5 | 3 | Chemie (CL) | |||
| 12 | Q (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 13 | R, S, T, U, V (A, T, P, D, U) | water | water | 18.0 | 400 | Chemie (HOH) |
Sequence modifications
A: 234 - 575 (UniProt: Q9UGP5)
| PDB | External Database | Details |
|---|---|---|
| Gly 230 | - | expression tag |
| Ser 231 | - | expression tag |
| Ala 232 | - | expression tag |
| Ala 233 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 45170.4 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 1016.3 | |
| All* | Total formula weight | 46186.7 |
