7M09
Pre-catalytic quaternary complex of DNA Polymerase Lambda with blunt-ended DSB substrate and incoming dUMPNPP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA polymerase lambda | polymer | 346 | 38551.0 | 1 | UniProt (Q9UGP5) Pfam (PF14716) Pfam (PF10391) Pfam (PF14792) Pfam (PF14791) | Homo sapiens (Human) | Pol Lambda,DNA polymerase beta-2,Pol beta2,DNA polymerase kappa |
| 2 | B (T) | DNA (5'-D(*CP*GP*GP*CP*A)-3') | polymer | 5 | 1505.0 | 1 | synthetic construct | ||
| 3 | C (P) | DNA (5'-D(*CP*AP*GP*TP*GP*C)-3') | polymer | 6 | 1809.2 | 1 | synthetic construct | ||
| 4 | D (D) | DNA (5'-D(P*GP*CP*CP*G)-3') | polymer | 4 | 1191.8 | 1 | synthetic construct | ||
| 5 | E (U) | DNA (5'-D(*GP*CP*AP*CP*TP*G)-3') | polymer | 6 | 1809.2 | 1 | synthetic construct | ||
| 6 | F (A) | 2'-DEOXYURIDINE 5'-ALPHA,BETA-IMIDO-TRIPHOSPHATE | non-polymer | 467.2 | 1 | Chemie (DUP) | |||
| 7 | G (A) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 8 | H, I (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 9 | J, K, N (A) | SODIUM ION | non-polymer | 23.0 | 3 | Chemie (NA) | |||
| 10 | L, M (A) | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
| 11 | O, P (A, P) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 12 | Q, R, S, T, U (A, T, P, D, U) | water | water | 18.0 | 442 | Chemie (HOH) |
Sequence modifications
A: 234 - 575 (UniProt: Q9UGP5)
| PDB | External Database | Details |
|---|---|---|
| Gly 230 | - | expression tag |
| Ser 231 | - | expression tag |
| Ala 232 | - | expression tag |
| Ala 233 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 44866.2 | |
| Non-Polymers* | Number of molecules | 11 |
| Total formula weight | 879.9 | |
| All* | Total formula weight | 45746.1 |
