7M08

Post-catalytic nicked complex of DNA Polymerase Lambda with bound 1-nt gapped SSB substrate and incoming dUMPNPP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA polymerase lambda	polymer	346	38551.0	1	UniProt (Q9UGP5) Pfam (PF14716) Pfam (PF10391) Pfam (PF14792) Pfam (PF14791) In PDB	Homo sapiens (Human)	Pol Lambda,DNA polymerase beta-2,Pol beta2,DNA polymerase kappa
2	T	DNA (5'-D(*CP*GP*GP*CP*AP*GP*TP*AP*CP*TP*G)-3')	polymer	11	3374.2	1		synthetic construct
3	P	DNA (5'-D(*CP*AP*GP*TP*AP*CP*T)-3')	polymer	7	2097.4	1		synthetic construct
4	D	DNA (5'-D(P*GP*CP*CP*G)-3')	polymer	4	1191.8	1		synthetic construct
5	A	SODIUM ION	non-polymer		23.0	3	Chemie (NA)
6	A	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
7	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
8	A	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
9	A	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
10		water	water		18.0	336	Chemie (HOH)

Sequence modifications

A: 234 - 575 (UniProt: Q9UGP5)

PDB	External Database	Details
Gly 230	-	expression tag
Ser 231	-	expression tag
Ala 232	-	expression tag
Ala 233	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	45214.4
Non-Polymers*	Number of molecules	8
	Total formula weight	285.3
All*	Total formula weight	45499.7

*Water molecules are not included.