7LYB

Cryo-EM structure of the human nucleosome core particle in complex with BRCA1-BARD1-UbcH5c

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, E)	Histone H3.1	polymer	140	15786.5	2	UniProt (P68431) Pfam (PF00125)	Homo sapiens (Human)	Histone H3/a,Histone H3/b,Histone H3/c,Histone H3/d,Histone H3/f,Histone H3/h,Histone H3/i,Histone H3/j,Histone H3/k,Histone H3/l
2	B, E (B, F)	Histone H4	polymer	107	11743.8	2	UniProt (P62805) Pfam (PF15511)	Homo sapiens (Human)
3	C, F (D, H)	Histone H2B type 1-J	polymer	126	13930.2	2	UniProt (P06899) Pfam (PF00125)	Homo sapiens (Human)	Histone H2B.1,Histone H2B.r,H2B/r
4	G (I)	DNA (147-MER)	polymer	147	45138.8	1		Homo sapiens (Human)
5	H (J)	DNA (146-MER)	polymer	147	45610.0	1		Homo sapiens (Human)
6	I (M)	Isoform 7 of Breast cancer type 1 susceptibility protein	polymer	124	13053.1	1	UniProt (P38398) Pfam (PF00097)	Homo sapiens (Human)	RING finger protein 53,RING-type E3 ubiquitin transferase BRCA1
7	J (P)	Ubiquitin-conjugating enzyme E2 D3	polymer	165	17869.1	1	UniProt (P61077) Pfam (PF00179)	Homo sapiens (Human)	(E3-independent) E2 ubiquitin-conjugating enzyme D3,E2 ubiquitin-conjugating enzyme D3,Ubiquitin carrier protein D3,Ubiquitin-conjugating enzyme E2(17)KB 3,Ubiquitin-conjugating enzyme E2-17 kDa 3,Ubiquitin-protein ligase D3
8	K (N)	BRCA1-associated RING domain protein 1	polymer	97	10906.6	1	UniProt (Q99728) Pfam (PF14835)	Homo sapiens (Human)	BARD-1,RING-type E3 ubiquitin transferase BARD1
9	L, M (C, G)	Histone H2A type 1-B/E	polymer	119	13034.2	2	UniProt (P04908) Pfam (PF00125) Pfam (PF16211)	Homo sapiens (Human)	Histone H2A.2,Histone H2A/a,Histone H2A/m
10	N, O, P, Q (M, N)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)

Sequence modifications

A, E: 0 - 135 (UniProt: P68431)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
His -1	-	expression tag

B, F: 0 - 102 (UniProt: P62805)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
His -1	-	expression tag

D, H: 0 - 123 (UniProt: P06899)

PDB	External Database	Details
Gly -2	-	expression tag
His -1	-	expression tag

M: 1 - 100 (UniProt: P38398)

PDB	External Database	Details
Gly -23	-	expression tag
Gly -22	-	expression tag
Ser -21	-	expression tag
Gly -20	-	expression tag
Gly -19	-	expression tag
Ser -18	-	expression tag
Gly -17	-	expression tag
Gly -16	-	expression tag
Ser -15	-	expression tag
Gly -14	-	expression tag
Gly -13	-	expression tag
Ser -12	-	expression tag
Gly -11	-	expression tag
Gly -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Gly -7	-	expression tag
Ser -6	-	expression tag
Gly -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

P: 2 - 147 (UniProt: P61077)

PDB	External Database	Details
Gly -17	-	expression tag
Gly -16	-	expression tag
Ser -15	-	expression tag
Gly -14	-	expression tag
Gly -13	-	expression tag
Ser -12	-	expression tag
Gly -11	-	expression tag
Gly -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Gly -7	-	expression tag
Ser -6	-	expression tag
Gly -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ser 1	-	expression tag

C, G: 12 - 129 (UniProt: P04908)

PDB	External Database	Details
Ser 11	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	13
	Total formula weight	241567.0
Non-Polymers*	Number of molecules	4
	Total formula weight	261.6
All*	Total formula weight	241828.6

*Water molecules are not included.