7LV6

The structure of MalL mutant enzyme S536R from Bacillus subtilis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Oligo-1,6-glucosidase 1	polymer	586	69305.6	1	UniProt (O06994) Pfam (PF00128) Pfam (PF16657)	Bacillus subtilis (strain 168)	Dextrin 6-alpha-D-glucanohydrolase,Oligosaccharide alpha-1,6-glucosidase 1,Sucrase-isomaltase 1,Isomaltase 1
2	B (B)	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	1	Chemie (TRS)
3	C (B)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4	D (B)	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
5	E (B)	water	water		18.0	785	Chemie (HOH)

Sequence modifications

B: 3 - 561 (UniProt: O06994)

PDB	External Database	Details
Met -24	-	expression tag
Ser -23	-	expression tag
Tyr -22	-	expression tag
Tyr -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
Asp -14	-	expression tag
Tyr -13	-	expression tag
Asp -12	-	expression tag
Ile -11	-	expression tag
Pro -10	-	expression tag
Thr -9	-	expression tag
Thr -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Ala 0	-	expression tag
Met 1	-	expression tag
Gly 2	-	expression tag
Arg 536	Ser 536	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	69305.6
Non-Polymers*	Number of molecules	3
	Total formula weight	254.3
All*	Total formula weight	69559.9

*Water molecules are not included.