7LRT

Cryo-EM structure of SARS-CoV-2 spike in complex with neutralizing antibody A23-58.1 that targets the receptor-binding domain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	F, H, D	antibody A23-58.1 heavy chain	polymer	228	24363.4	3		Homo sapiens (Human)
2	G, L, E	antibody A23-58.1 light chain	polymer	215	23475.0	3		Homo sapiens (Human)
3	A, B, C	SARS-CoV-2 spike glycoprotein	polymer	1195	132334.5	3	UniProt (P0DTC2) Pfam (PF16451) Pfam (PF09408) Pfam (PF19209) Pfam (PF01601) In PDB	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV SARS-CoV-2)	S glycoprotein,E2,Peplomer protein
4	I, J, K, Z	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	4	In PDB GlyTouCan (G15407YE)
5	a, b, c, d, M...	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	15	In PDB GlyTouCan (G42666HT)
6	e, S, Y	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	3	In PDB GlyTouCan (G22768VO)
7	C, A, B	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	27	Chemie (NAG)

Sequence modifications

A, B, C: 14 - 1208 (UniProt: P0DTC2)

PDB	External Database	Details
Gly 682	Arg 682	conflict
Ser 683	Arg 683	conflict
Ser 685	Arg 685	conflict
Pro 817	Phe 817	conflict
Pro 892	Ala 892	conflict
Pro 899	Ala 899	conflict
Pro 942	Ala 942	conflict
Pro 986	Lys 986	conflict
Pro 987	Val 987	conflict

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	540518.7
Branched	Number of molecules	22
	Total formula weight	11444.7
Non-Polymers*	Number of molecules	27
	Total formula weight	5972.6
All*	Total formula weight	557936.0

*Water molecules are not included.