7LR8

Crystal structure of GH5_18-E153A from Streptomyces cattleya in complex with Manb1-4GlcNAc

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Uncharacterized protein ScGH5_18	polymer	433	47858.3	2	UniProt (F8JJ04) In PDB	Streptomyces cattleya (strain ATCC 35852 / DSM 46488 / JCM 4925 / NBRC 14057 / NRRL 8057)
2	E, C	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		383.3	2	In PDB GlyTouCan (G90333CG)
3	A, B	2-(2-ETHOXYETHOXY)ETHANOL	non-polymer		134.2	3	Chemie (AE3)
4	B	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
5	B	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
6		water	water		18.0	829	Chemie (HOH)

Sequence modifications

A, B: 1 - 425 (UniProt: F8JJ04)

PDB	External Database	Details
Ala 153	Glu 153	engineered mutation
Leu 426	-	expression tag
Glu 427	-	expression tag
His 428	-	expression tag
His 429	-	expression tag
His 430	-	expression tag
His 431	-	expression tag
His 432	-	expression tag
His 433	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	95716.7
Branched	Number of molecules	2
	Total formula weight	766.7
Non-Polymers*	Number of molecules	7
	Total formula weight	752.8
All*	Total formula weight	97236.2

*Water molecules are not included.