7LOF

T4 lysozyme mutant L99A in complex with 2-butylthiophene

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Lysozyme	polymer	172	19691.5	1	UniProt (D9IEF7) Pfam (PF00959) UniProt (by SIFTS) (P00720) In PDB	Enterobacteria phage T4 (Bacteriophage T4)	Endolysin,Lysis protein,Muramidase
2	A	2-butylthiophene	non-polymer		140.2	1	Chemie (Y8G)
3	A	BETA-MERCAPTOETHANOL	non-polymer		78.1	1	Chemie (BME)
4	A	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	1	Chemie (TRS)
5		water	water		18.0	116	Chemie (HOH)

Sequence modifications

A: 1 - 164 (UniProt: D9IEF7)

PDB	External Database	Details
Ala 99	Leu 99	engineered mutation
Leu 165	-	expression tag
Glu 166	-	expression tag
His 167	-	expression tag
His 168	-	expression tag
His 169	-	expression tag
His 170	-	expression tag
His 171	-	expression tag
His 172	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	19691.5
Non-Polymers*	Number of molecules	3
	Total formula weight	340.5
All*	Total formula weight	20032.1

*Water molecules are not included.