7LNW

I146A mutant of the isopentenyl phosphate kinase from Candidatus methanomethylophilus alvus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Isopentenyl phosphate kinase	polymer	279	30491.1	2	UniProt (A0A3G3II74) Pfam (PF00696) UniProt (by SIFTS) (M9SEQ1)	Candidatus Methanomethylophilus alvus	IPK
2	C, E (A, B)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)
3	D, F (A, B)	[(~{Z})-5-[(azanylidene-$l^{4}-azanylidene)amino]-3-methyl-pent-2-enyl] dihydrogen phosphate	non-polymer		222.2	2	Chemie (Y77)
4	G, H (A, B)	water	water		18.0	83	Chemie (HOH)

Sequence modifications

A, B: 1 - 259 (UniProt: A0A3G3II74)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 146	Ile 146	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	60982.3
Non-Polymers*	Number of molecules	4
	Total formula weight	1298.7
All*	Total formula weight	62281.0

*Water molecules are not included.