7LLK

Cryo-EM structure of Q23.17_DS-SOSIP in complex with Glycan276-Dependent Broadly Neutralizing Antibody 179NC75 Fab

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	E, A, I	Envelope glycoprotein gp120	polymer	474	53627.9	3	UniProt (O55774) Pfam (PF00516) In PDB	Human immunodeficiency virus 1
2	F, B, J	Envelope glycoprotein gp41	polymer	153	17211.6	3	Pfam (PF00517) UniProt (by SIFTS) (O55774) In PDB	Human immunodeficiency virus 1
3	G, C, K	179NC75 Fab Heavy chain	polymer	238	25897.5	3		Homo sapiens (Human)
4	H, D, L	179NC75 Fab Light chain	polymer	215	23042.4	3		Homo sapiens (Human)
5	f, M, q, V	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	4	In PDB GlyTouCan (G42666HT)
6	a, b, d, e, g...	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	22	In PDB GlyTouCan (G15407YE)
7	c, h, n, s, S...	alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	6	In PDB GlyTouCan (G22573RC)
8	I, E, A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	10	Chemie (NAG)

Sequence modifications

E, A, I: 32 - 508 (UniProt: O55774)

PDB	External Database	Details
Ala 31	-	expression tag
Cys 201	Ile 193	conflict
Ala 219	Thr 211	conflict
Ser 334	Thr 325	conflict
Cys 433	Ala 423	conflict
Lys 490	Glu 480	conflict
Cys 501	Ala 491	conflict
Arg 509	-	expression tag
Arg 510	-	expression tag
Arg 511	-	expression tag
Arg 512	-	expression tag
Arg 513	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	359338.1
Branched	Number of molecules	32
	Total formula weight	19093.6
Non-Polymers*	Number of molecules	10
	Total formula weight	2212.1
All*	Total formula weight	380643.8

*Water molecules are not included.