7LFQ

Pyrococcus RNA ligase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	RNA-splicing ligase RtcB	polymer	501	55801.8	1	UniProt (O59245) Pfam (PF01139)	Pyrococcus horikoshii	3'-phosphate/5'-hydroxy nucleic acid ligase
2	B (B)	DNA (5'-D(*AP*TP*GP*TP*CP*C)-3')	polymer	6	1784.2	1		synthetic construct
3	C (A)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
4	D (A)	POTASSIUM ION	non-polymer		39.1	1	Chemie (K)
5	E (A)	water	water		18.0	17	Chemie (HOH)

Sequence modifications

A: 1 - 96 (UniProt: O59245)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

A: 97 - 481 (UniProt: O59245)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	57586.0
Non-Polymers*	Number of molecules	2
	Total formula weight	135.2
All*	Total formula weight	57721.1

*Water molecules are not included.