7LEN

Crystal structure of the epidermal growth factor receptor extracellular region with R84K mutation in complex with epiregulin crystallized with trehalose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Isoform 4 of Epidermal growth factor receptor	polymer	507	56494.2	2	UniProt (P00533) Pfam (PF01030) Pfam (PF00757)	Homo sapiens (Human)	Proto-oncogene c-ErbB-1,Receptor tyrosine-protein kinase erbB-1
2	C, D (C, D)	Proepiregulin	polymer	48	5480.3	2	UniProt (O14944)	Homo sapiens (Human)
3	E, I (E, I)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
4	F (F)	alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	1	In PDB GlyTouCan (G81315DD)
5	G (G)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G35932AR)
6	H (H)	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	1	In PDB GlyTouCan (G22768VO)
7	J, K (A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)

Sequence modifications

A, B: 1 - 501 (UniProt: P00533)

PDB	External Database	Details
Lys 84	Arg 108	engineered mutation
His 502	-	expression tag
His 503	-	expression tag
His 504	-	expression tag
His 505	-	expression tag
His 506	-	expression tag
His 507	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	123949.0
Branched	Number of molecules	5
	Total formula weight	2932.7
Non-Polymers*	Number of molecules	2
	Total formula weight	442.4
All*	Total formula weight	127324.1

*Water molecules are not included.