7LDU

Zoogloea ramigera biosynthetic thiolase Q183Y/Y218E/delH221 mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Acetyl-CoA acetyltransferase	polymer	398	41406.2	4	UniProt (P07097) Pfam (PF00108) Pfam (PF02803)	Zoogloea ramigera	Acetoacetyl-CoA thiolase,Beta-ketothiolase
2	E, F, G, I, J... (A, B)	SULFATE ION	non-polymer		96.1	8	Chemie (SO4)
3	H, O, Q, R (A, B, C, D)	COENZYME A	non-polymer		767.5	4	Chemie (COA)
4	K, P (A, B)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
5	S, T, U, V (A, B, C, D)	water	water		18.0	187	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 391 (UniProt: P07097)

PDB	External Database	Details
Met -6	-	initiating methionine
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Ala 11	-	insertion
Arg 129	Ala 129	conflict
Tyr 183	Gln 183	engineered mutation
Glu 218	Tyr 218	engineered mutation
-	His 221	deletion

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	165624.6
Non-Polymers*	Number of molecules	14
	Total formula weight	3909.5
All*	Total formula weight	169534.2

*Water molecules are not included.