7LDT

Zoogloea ramigera biosynthetic thiolase Q183Y/Y218E mutant, RbCl soak

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Acetyl-CoA acetyltransferase	polymer	399	41544.3	4	UniProt (P07097) Pfam (PF00108) Pfam (PF02803)	Zoogloea ramigera	Acetoacetyl-CoA thiolase,Beta-ketothiolase
2	E, F, I, M (A, B, C)	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
3	G, J, L, O (A, B, C, D)	COENZYME A	non-polymer		767.5	4	Chemie (COA)
4	H, K, N, P (A, B, C, D)	RUBIDIUM ION	non-polymer		85.5	4	Chemie (RB)
5	Q, R, S, T (A, B, C, D)	water	water		18.0	868	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 392 (UniProt: P07097)

PDB	External Database	Details
Met -6	-	initiating methionine
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Ala 11	-	insertion
Arg 129	Ala 129	conflict
Tyr 183	Gln 183	engineered mutation
Glu 218	Tyr 218	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	166177.2
Non-Polymers*	Number of molecules	12
	Total formula weight	3796.3
All*	Total formula weight	169973.5

*Water molecules are not included.