7LD2

Zoogloea ramigera biosynthetic thiolase Q183Y mutant, RbCl soak

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Acetyl-CoA acetyltransferase	polymer	399	41578.4	4	UniProt (P07097) Pfam (PF00108) Pfam (PF02803)	Zoogloea ramigera	Acetoacetyl-CoA thiolase,Beta-ketothiolase
2	E, F, O, P (A, B)	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
3	G, Q, W, Y (A, B, C, D)	COENZYME A	non-polymer		767.5	4	Chemie (COA)
4	H, R, X, Z (A, B, C, D)	RUBIDIUM ION	non-polymer		85.5	4	Chemie (RB)
5	I, J, K, L, M... (A, B)	CHLORIDE ION	non-polymer		35.5	10	Chemie (CL)
6	AA, BA, CA, DA (A, B, C, D)	water	water		18.0	511	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 392 (UniProt: P07097)

PDB	External Database	Details
Met -6	-	initiating methionine
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Ala 11	-	insertion
Arg 129	Ala 129	conflict
Tyr 183	Gln 183	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	166313.5
Non-Polymers*	Number of molecules	22
	Total formula weight	4150.8
All*	Total formula weight	170464.3

*Water molecules are not included.