7LCL

Zoogloea ramigera biosynthetic thiolase Q183Y mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Acetyl-CoA acetyltransferase	polymer	399	41578.4	4	UniProt (P07097) Pfam (PF00108) Pfam (PF02803) In PDB	Zoogloea ramigera	Acetoacetyl-CoA thiolase,Beta-ketothiolase
2	A, B, C	SULFATE ION	non-polymer		96.1	11	Chemie (SO4)
3	A, B, C, D	COENZYME A	non-polymer		767.5	4	Chemie (COA)
4	A, B, C, D	AMMONIUM ION	non-polymer		18.0	4	Chemie (NH4)
5		water	water		18.0	1020	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 392 (UniProt: P07097)

PDB	External Database	Details
Met -6	-	initiating methionine
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Ala 11	-	insertion
Arg 129	Ala 129	conflict
Tyr 183	Gln 183	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	166313.5
Non-Polymers*	Number of molecules	19
	Total formula weight	4199.0
All*	Total formula weight	170512.4

*Water molecules are not included.