7LC2

Crystal Structure of KRAS4b-Q61R (GMPPNP-bound) in complex with the RAS-binding domain (RBD) of SIN1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	GTPase KRas	polymer	170	19545.5	2	UniProt (P01116) Pfam (PF00071) In PDB	Homo sapiens (Human)	K-Ras 2,Ki-Ras,c-K-ras,c-Ki-ras
2	D, E	Target of rapamycin complex 2 subunit MAPKAP1	polymer	88	9824.1	2	UniProt (Q9BPZ7) In PDB	Homo sapiens (Human)	TORC2 subunit MAPKAP1,Mitogen-activated protein kinase 2-associated protein 1,Stress-activated map kinase-interacting protein 1,mSIN1
3	A, B	PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER	non-polymer		522.2	2	Chemie (GNP)
4	A, B	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5		water	water		18.0	23	Chemie (HOH)

Sequence modifications

A, B: 1 - 169 (UniProt: P01116)

PDB	External Database	Details
Gly 0	-	expression tag
Arg 61	Gln 61	engineered mutation

D, E: 275 - 361 (UniProt: Q9BPZ7)

PDB	External Database	Details
Gly 274	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	58739.1
Non-Polymers*	Number of molecules	4
	Total formula weight	1093.0
All*	Total formula weight	59832.1

*Water molecules are not included.