7L6O

Cryo-EM structure of HIV-1 Env CH848.3.D0949.10.17chim.6R.DS.SOSIP.664

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	a, c, e	CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 - gp120	polymer	466	52061.9	3	UniProt (A0A1W6IPB2) Pfam (PF00516) In PDB	Human immunodeficiency virus 1 (HIV-1)	Envelope glycoprotein gp120
2	b, d, f	CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 - gp41	polymer	146	16578.8	3	Pfam (PF00517) UniProt (by SIFTS) (Q2N0S6) In PDB	Human immunodeficiency virus 1
3	V, W, Y, Z, A...	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	27	In PDB GlyTouCan (G15407YE)
4	U, X, C, i, H...	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	9	In PDB GlyTouCan (G42666HT)
5	E, k, R	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	3	In PDB GlyTouCan (G22768VO)
6	c, d, e, f, a...	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	18	Chemie (NAG)

Sequence modifications

a, c, e: 34 - 506 (UniProt: A0A1W6IPB2)

PDB	External Database	Details
Ala 31	-	expression tag
Glu 32	-	expression tag
Asn 33	-	expression tag
Cys 201	Val 189	engineered mutation
Cys 433	Ala 417	engineered mutation
Lys 490	Glu 474	engineered mutation
Glu 492	Gln 476	engineered mutation
Val 496	Ile 480	engineered mutation
Arg 500	Gly 484	engineered mutation
Cys 501	Ala 485	engineered mutation
Gly 506	Glu 491	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	205922.2
Branched	Number of molecules	39
	Total formula weight	22388.7
Non-Polymers*	Number of molecules	18
	Total formula weight	3981.7
All*	Total formula weight	232292.7

*Water molecules are not included.