7L4I

Crystal structure of a substrate-trapping variant of PPM1H phosphatase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Protein phosphatase 1H	polymer	451	50318.0	2	UniProt (Q9ULR3) Pfam (PF00481) In PDB	Homo sapiens (Human)
2	A, B	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
3		water	water		18.0	91	Chemie (HOH)

Sequence modifications

A, B: 33 - 222 (UniProt: Q9ULR3)

PDB	External Database	Details
Gly 29	-	expression tag
Ser 30	-	expression tag
His 31	-	expression tag
Met 32	-	expression tag
Ala 56	Cys 56	engineered mutation

A, B: 227 - 514 (UniProt: Q9ULR3)

PDB	External Database	Details
Gly 223	-	linker
Ser 224	-	linker
Gly 225	-	linker
Ser 226	-	linker
Ala 288	Asp 288	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	100635.9
Non-Polymers*	Number of molecules	4
	Total formula weight	97.2
All*	Total formula weight	100733.1

*Water molecules are not included.