7L31
Cyro-EM structure of human Glycine Receptor alpha2-beta heteromer, strychnine bound state, 3.8 Angstrom
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Glycine receptor subunit alpha-2 | polymer | 364 | 41676.0 | 4 | UniProt (P23416) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (Human) | |
| 2 | E (E) | Glycine receptor subunit beta,Green fluorescent protein | polymer | 702 | 78765.1 | 1 | UniProt (P48167) UniProt (P42212) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (Human) | Glycine receptor 58 kDa subunit |
| 3 | F, G, I, J, L... (A, B, C, D, E) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 9 | Chemie (NAG) | |||
| 4 | H, K, N, Q, R (A, B, C, D) | STRYCHNINE | non-polymer | 334.4 | 5 | Chemie (SY9) PubChem (5304) PubChem (121457) PubChem (3035321) PubChem (441071) PubChem (3037422) PubChem (3083694) PubChem (5321532) PubChem (6604670) PubChem (11336581) PubChem (12359078) PubChem (16757746) PubChem (16759292) PubChem (16760012) PubChem (23423987) PubChem (23636622) PubChem (24011700) PubChem (25022186) PubChem (44135720) PubChem (51035301) PubChem (68315627) PubChem (5979) PubChem (3246505) PubChem (98049551) PubChem (5702135) PubChem (5705187) PubChem (54235484) PubChem (9840646) PubChem (9997016) PubChem (133611800) PubChem (71057032) PubChem (71073285) PubChem (74892805) PubChem (75539564) PubChem (98049553) PubChem (100078880) PubChem (124352578) PubChem (124389223) PubChem (124803013) PubChem (134814445) PubChem (134944247) PubChem (142877741) PubChem (149063339) PubChem (155935641) PubChem (162987392) PubChem (162987393) PubChem (162987394) PubChem (162987395) PubChem (162987396) PubChem (173470783) |
Sequence modifications
A, B, C, D: 1 - 316 (UniProt: P23416)
A, B, C, D: 382 - 425 (UniProt: P23416)
E: 3 - 331 (UniProt: P48167)
E: 331 - 340 (UniProt: P42212)
E: 340 - 475 (UniProt: P48167)
A, B, C, D: 382 - 425 (UniProt: P23416)
| PDB | External Database | Details |
|---|---|---|
| Gly 378 | - | linker |
| Ser 379 | - | linker |
| Ser 380 | - | linker |
| Gly 381 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Gly -19 | - | expression tag |
| Val -18 | - | expression tag |
| Ala -17 | - | expression tag |
| Met -16 | - | expression tag |
| Pro -15 | - | expression tag |
| Gly -14 | - | expression tag |
| Ala -13 | - | expression tag |
| Glu -12 | - | expression tag |
| Asp -11 | - | expression tag |
| Asp -10 | - | expression tag |
| Val -9 | - | expression tag |
| Val -8 | - | expression tag |
| Ala -7 | - | expression tag |
| Ala -6 | - | expression tag |
| Leu -5 | - | expression tag |
| Glu -4 | - | expression tag |
| Val -3 | - | expression tag |
| Leu -2 | - | expression tag |
| Phe -1 | - | expression tag |
| Gln 0 | - | expression tag |
| Gly 1 | - | expression tag |
| Pro 2 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Leu 333 | Phe 64 | engineered mutation |
| Thr 333 | Ser 65 | engineered mutation |
| Lys 339 | Ala 206 | engineered mutation |
| Leu 340 | His 231 | engineered mutation |
| Ser 340 | - | linker |
| Gly 340 | - | linker |
| Ser 340 | - | linker |
| Gly 340 | - | linker |
| Ser 340 | - | linker |
| Gly 340 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Leu 331 | - | linker |
| Asn 331 | - | linker |
| Gly 331 | - | linker |
| Gly 331 | - | linker |
| Ser 331 | - | linker |
| Ser 331 | - | linker |
| Ala 331 | - | linker |
| Ala 331 | - | linker |
| Ala 331 | - | linker |
| Val 331 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 245469.1 | |
| Non-Polymers* | Number of molecules | 14 |
| Total formula weight | 3662.9 | |
| All* | Total formula weight | 249132.0 |
