7L0S

Structure of NTS-NTSR1-Gi complex in lipid nanodisc, noncanonical state, with AHD

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (C)	Neurotensin receptor type 1	polymer	336	37483.0	1	UniProt (P20789) Pfam (PF00001)	Rattus norvegicus (Rat)	NTR1,High-affinity levocabastine-insensitive neurotensin receptor,NTRH
2	B (D)	Neurotensin	polymer	10	1087.3	1	UniProt (P20068)	Rattus norvegicus (Rat)
3	C (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40415.0	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (Human)	Adenylate cyclase-inhibiting G alpha protein
4	D (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	361	39983.7	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (Human)	Transducin beta chain 1
5	E (G)	Guanine nucleotide-binding protein G(T) subunit gamma-T1	polymer	83	9593.0	1	UniProt (P63211) Pfam (PF00631)	Homo sapiens (Human)	Transducin gamma chain

Sequence modifications

C: 50 - 390 (UniProt: P20789)

PDB	External Database	Details
Gly 46	-	expression tag
Pro 47	-	expression tag
Gly 48	-	expression tag
Ser 49	-	expression tag
Leu 86	Ala 86	engineered mutation
Asp 103	His 103	engineered mutation
Tyr 105	His 105	engineered mutation
Val 161	Ala 161	engineered mutation
Leu 213	Arg 213	engineered mutation
Leu 234	Val 234	engineered mutation
Ala 253	Ile 253	engineered mutation
-	Glu 273	deletion
-	Gln 274	deletion
-	Gly 275	deletion
-	Arg 276	deletion
-	Val 277	deletion
-	Cys 278	deletion
-	Thr 279	deletion
-	Val 280	deletion
-	Gly 281	deletion
-	Thr 282	deletion
-	His 283	deletion
-	Asn 284	deletion
-	Gly 285	deletion
-	Leu 286	deletion
-	Glu 287	deletion
-	His 288	deletion
-	Ser 289	deletion
-	Thr 290	deletion
Arg 305	His 305	engineered mutation
Val 358	Phe 358	engineered mutation
Ala 362	Ser 362	engineered mutation
Thr 391	-	expression tag
Arg 392	-	expression tag
Glu 393	-	expression tag
Leu 394	-	expression tag
Glu 395	-	expression tag
Val 396	-	expression tag
Leu 397	-	expression tag
Phe 398	-	expression tag
Gln 399	-	expression tag

D: 8 - 13 (UniProt: P20068)

PDB	External Database	Details
Gly 4	-	expression tag
Pro 5	-	expression tag
Gly 6	-	expression tag
Gly 7	-	expression tag

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -20	-	initiating methionine
Arg -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Leu -6	-	expression tag
Glu -5	-	expression tag
Val -4	-	expression tag
Leu -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag
Pro 1	-	expression tag

G: 2 - 74 (UniProt: P63211)

PDB	External Database	Details
Met -8	-	initiating methionine
Tyr -7	-	expression tag
Pro -6	-	expression tag
Tyr -5	-	expression tag
Asp -4	-	expression tag
Val -3	-	expression tag
Pro -2	-	expression tag
Asp -1	-	expression tag
Tyr 0	-	expression tag
Ala 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	128562.1
All*	Total formula weight	128562.1

*Water molecules are not included.