7KZS

Structure of the human fanconi anaemia Core-UBE2T-ID-DNA complex in open state

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, L (A, S)	Fanconi anemia group A protein	polymer	1477	165513.0	2	UniProt (O15360) Pfam (PF15865) Pfam (PF24781) Pfam (PF24783) Pfam (PF03511)	Homo sapiens (Human)	Protein FACA
2	B, I (B, O)	Fanconi anemia group B protein	polymer	884	100640.2	2	UniProt (Q8NB91)	Homo sapiens (Human)	Protein FACB,Fanconi anemia-associated polypeptide of 95 kDa,FAAP95
3	C (C)	Fanconi anemia group C protein	polymer	583	66290.4	1	UniProt (Q00597) Pfam (PF02106)	Homo sapiens (Human)	Protein FACC
4	D (E)	Fanconi anemia group E protein	polymer	555	61026.1	1	UniProt (Q9HB96) Pfam (PF11510)	Homo sapiens (Human)	Protein FACE
5	E (F)	Fanconi anemia group F protein	polymer	399	45108.3	1	UniProt (Q9NPI8) Pfam (PF11107)	Homo sapiens (Human)	Protein FACF
6	F, G (G, H)	Fanconi anemia group G protein	polymer	641	70873.7	2	UniProt (O15287)	Homo sapiens (Human)	Protein FACG,DNA repair protein XRCC9
7	H, N (L, M)	E3 ubiquitin-protein ligase FANCL	polymer	394	45204.0	2	UniProt (Q9NW38) Pfam (PF18891) Pfam (PF11793) Pfam (PF09765) Pfam (PF18890)	Homo sapiens (Human)	Fanconi anemia group L protein,Fanconi anemia-associated polypeptide of 43 kDa,FAAP43,RING-type E3 ubiquitin transferase FANCL
8	J, K (P, Q)	Fanconi anemia core complex-associated protein 100	polymer	906	96513.9	2	UniProt (Q0VG06) Pfam (PF15146)	Homo sapiens (Human)	Fanconi anemia-associated protein of 100 kDa
9	M (W)	Fanconi anemia core complex-associated protein 20	polymer	39	4041.7	1		Homo sapiens (human)
10	O (X)	Ubiquitin-conjugating enzyme E2 T	polymer	197	22553.9	1	UniProt (Q9NPD8) Pfam (PF00179)	Homo sapiens (Human)	Cell proliferation-inducing gene 50 protein,E2 ubiquitin-conjugating enzyme T,Ubiquitin carrier protein T,Ubiquitin-protein ligase T
11	P (U)	Fanconi anemia, complementation group I	polymer	1328	149512.1	1	UniProt (B7ZMF2)	Homo sapiens (Human)
12	Q (V)	Fanconi anemia group D2 protein	polymer	1451	164325.5	1	UniProt (Q9BXW9) Pfam (PF14631)	Homo sapiens (Human)	Protein FACD2
13	R (Y)	DNA (25-MER)	polymer	58	18036.5	1		synthetic construct
14	S (Z)	DNA (25-MER)	polymer	58	17716.3	1		synthetic construct
15	T, U, V, W, X (G, L, M)	ZINC ION	non-polymer		65.4	5	Chemie (ZN)

Sequence modifications

A, S: 1 - 1455 (UniProt: O15360)

PDB	External Database	Details
Ala 1456	-	expression tag
Ala 1457	-	expression tag
Ala 1458	-	expression tag
Lys 1459	-	expression tag
Leu 1460	-	expression tag
Val 1461	-	expression tag
Asp 1462	-	expression tag
Glu 1463	-	expression tag
Asp 1464	-	expression tag
Leu 1465	-	expression tag
Tyr 1466	-	expression tag
Phe 1467	-	expression tag
Gln 1468	-	expression tag
Ser 1469	-	expression tag
Asp 1470	-	expression tag
Tyr 1471	-	expression tag
Lys 1472	-	expression tag
Asp 1473	-	expression tag
Asp 1474	-	expression tag
Asp 1475	-	expression tag
Asp 1476	-	expression tag
Lys 1477	-	expression tag

B, O: 1 - 859 (UniProt: Q8NB91)

PDB	External Database	Details
Met -24	-	initiating methionine
Asp -23	-	expression tag
Tyr -22	-	expression tag
Lys -21	-	expression tag
Asp -20	-	expression tag
Asp -19	-	expression tag
Asp -18	-	expression tag
Asp -17	-	expression tag
Lys -16	-	expression tag
Glu -15	-	expression tag
Asn -14	-	expression tag
Leu -13	-	expression tag
Tyr -12	-	expression tag
Phe -11	-	expression tag
Gln -10	-	expression tag
Gly -9	-	expression tag
Gly -8	-	expression tag
Gly -7	-	expression tag
Arg -6	-	expression tag
Lys -5	-	expression tag
Leu -4	-	expression tag
Gly -3	-	expression tag
Thr -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

C: 1 - 558 (UniProt: Q00597)

PDB	External Database	Details
Met -24	-	initiating methionine
Asp -23	-	expression tag
Tyr -22	-	expression tag
Lys -21	-	expression tag
Asp -20	-	expression tag
Asp -19	-	expression tag
Asp -18	-	expression tag
Asp -17	-	expression tag
Lys -16	-	expression tag
Glu -15	-	expression tag
Asn -14	-	expression tag
Leu -13	-	expression tag
Tyr -12	-	expression tag
Phe -11	-	expression tag
Gln -10	-	expression tag
Gly -9	-	expression tag
Gly -8	-	expression tag
Gly -7	-	expression tag
Arg -6	-	expression tag
Lys -5	-	expression tag
Leu -4	-	expression tag
Gly -3	-	expression tag
Thr -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

E: 1 - 536 (UniProt: Q9HB96)

PDB	External Database	Details
Met -18	-	initiating methionine
Asp -17	-	expression tag
Tyr -16	-	expression tag
Lys -15	-	expression tag
Asp -14	-	expression tag
Asp -13	-	expression tag
Asp -12	-	expression tag
Asp -11	-	expression tag
Lys -10	-	expression tag
Glu -9	-	expression tag
Asn -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag

F: 1 - 374 (UniProt: Q9NPI8)

PDB	External Database	Details
Met -24	-	initiating methionine
Asp -23	-	expression tag
Tyr -22	-	expression tag
Lys -21	-	expression tag
Asp -20	-	expression tag
Asp -19	-	expression tag
Asp -18	-	expression tag
Asp -17	-	expression tag
Lys -16	-	expression tag
Glu -15	-	expression tag
Asn -14	-	expression tag
Leu -13	-	expression tag
Tyr -12	-	expression tag
Phe -11	-	expression tag
Gln -10	-	expression tag
Gly -9	-	expression tag
Gly -8	-	expression tag
Gly -7	-	expression tag
Arg -6	-	expression tag
Lys -5	-	expression tag
Leu -4	-	expression tag
Gly -3	-	expression tag
Thr -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

G, H: 1 - 622 (UniProt: O15287)

PDB	External Database	Details
Met -18	-	initiating methionine
Asp -17	-	expression tag
Tyr -16	-	expression tag
Lys -15	-	expression tag
Asp -14	-	expression tag
Asp -13	-	expression tag
Asp -12	-	expression tag
Asp -11	-	expression tag
Lys -10	-	expression tag
Glu -9	-	expression tag
Asn -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag

L, M: 1 - 375 (UniProt: Q9NW38)

PDB	External Database	Details
Met -18	-	initiating methionine
Asp -17	-	expression tag
Tyr -16	-	expression tag
Lys -15	-	expression tag
Asp -14	-	expression tag
Asp -13	-	expression tag
Asp -12	-	expression tag
Asp -11	-	expression tag
Lys -10	-	expression tag
Glu -9	-	expression tag
Asn -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag

P, Q: 1 - 881 (UniProt: Q0VG06)

PDB	External Database	Details
Met -24	-	initiating methionine
Asp -23	-	expression tag
Tyr -22	-	expression tag
Lys -21	-	expression tag
Asp -20	-	expression tag
His -19	-	expression tag
Asp -18	-	expression tag
Gly -17	-	expression tag
Asp -16	-	expression tag
Tyr -15	-	expression tag
Lys -14	-	expression tag
Asp -13	-	expression tag
His -12	-	expression tag
Asp -11	-	expression tag
Ile -10	-	expression tag
Asp -9	-	expression tag
Tyr -8	-	expression tag
Lys -7	-	expression tag
Asp -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Lys -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

U: 1 - 1328 (UniProt: B7ZMF2)

PDB	External Database	Details
Leu 877	Ile 877	conflict
Val 1235	Ala 1235	conflict
Ser 1274	Asn 1274	conflict

Contents of the asymmetric unit

Polymers	Number of chains	19
	Total formula weight	1506100.3
Non-Polymers*	Number of molecules	5
	Total formula weight	327.0
All*	Total formula weight	1506427.3

*Water molecules are not included.