7KZ2

Dihydrodipicolinate synthase (DHDPS) from C.jejuni, Y110F mutant with pyruvate bound in the active site and L-lysine bound at the allosteric site in C2221 space group

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	4-hydroxy-tetrahydrodipicolinate synthase	polymer	310	34169.3	6	UniProt (Q9PPB4) Pfam (PF00701)	Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)	HTPA synthase
2	FA, G, PA, SA, V... (C, A, D, E, B...)	LYSINE	non-polymer		147.2	6	Chemie (LYS)
3	GA, H, HA, I, J... (C, A, E, B, F)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	13	Chemie (PEG)
4	AA, AB, IA, JA, KA... (B, F, C, A, D...)	1,2-ETHANEDIOL	non-polymer		62.1	17	Chemie (EDO)
5	BB, DA, S, T (F, B, A)	ACETATE ION	non-polymer		59.0	4	Chemie (ACT)
6	CB, DB, EA, OA, RA... (F, B, C, D, A...)	TRIETHYLENE GLYCOL	non-polymer		150.2	7	Chemie (PGE)
7	BA, CA, NA (B, C)	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
8	EB, FB, GB, HB, IB... (A, B, C, D, E...)	water	water		18.0	1099	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 1 - 298 (UniProt: Q9PPB4)

PDB	External Database	Details
Met -11	-	expression tag
Arg -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Phe 110	Tyr 110	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	205015.5
Non-Polymers*	Number of molecules	50
	Total formula weight	4678.2
All*	Total formula weight	209693.7

*Water molecules are not included.