7KXS
Computational design of constitutively active cGAS
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Cyclic GMP-AMP synthase | polymer | 362 | 42414.1 | 2 | UniProt (Q8C6L5) Pfam (PF03281) Pfam (PF20266) | Mus musculus | m-cGAS,2'3'-cGAMP synthase,Mab-21 domain-containing protein 1 |
| 2 | C, D (A, B) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 3 | E, F (A, B) | water | water | 18.0 | 36 | Chemie (HOH) |
Sequence modifications
A, B: 147 - 507 (UniProt: Q8C6L5)
| PDB | External Database | Details |
|---|---|---|
| Ser 146 | - | expression tag |
| Asp 160 | Lys 160 | engineered mutation |
| Pro 161 | Arg 161 | engineered mutation |
| Phe 196 | Asn 196 | engineered mutation |
| Val 197 | Thr 197 | engineered mutation |
| Ile 200 | Tyr 200 | engineered mutation |
| Lys 203 | His 203 | engineered mutation |
| Met 395 | Lys 395 | engineered mutation |
| Met 399 | Lys 399 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 84828.2 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 130.8 | |
| All* | Total formula weight | 84959.0 |
