7KUZ

Dihydrodipicolinate synthase (DHDPS) from C.jejuni, H56N mutant with pyruvate bound in the active site and L-lysine bound at the allosteric site

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, F (A, B, C, D, F)	4-hydroxy-tetrahydrodipicolinate synthase	polymer	310	34161.2	5	UniProt (Q9PPB4) Pfam (PF00701)	Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)	HTPA synthase
2	E (E)	4-hydroxy-tetrahydrodipicolinate synthase	polymer	310	34092.2	1	UniProt (Q9PPB4) Pfam (PF00701)	Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)	HTPA synthase
3	G, HB, NA, T, U... (A, F, D, B, E)	LYSINE	non-polymer		147.2	6	Chemie (LYS)
4	AB, GA, H, HA, IB... (E, C, A, F, B)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	10	Chemie (PEG)
5	AA, BB, CB, I, IA... (B, E, A, C, F...)	1,2-ETHANEDIOL	non-polymer		62.1	21	Chemie (EDO)
6	BA, CA, DA, DB, EA... (B, E, C, A, F...)	ACETATE ION	non-polymer		59.0	9	Chemie (ACT)
7	EB, FA, FB, LA, N... (E, B, C, A, F...)	MAGNESIUM ION	non-polymer		24.3	12	Chemie (MG)
8	GB, MA, Q, R, WA (E, C, A, D)	TRIETHYLENE GLYCOL	non-polymer		150.2	5	Chemie (PGE)
9	S (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
10	SB, TB, UB, VB, WB... (A, B, C, D, E...)	water	water		18.0	1113	Chemie (HOH)

Sequence modifications

A, B, C, D, F: 1 - 298 (UniProt: Q9PPB4)

PDB	External Database	Details
Met -11	-	expression tag
Arg -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Asn 56	His 56	engineered mutation

E: 1 - 298 (UniProt: Q9PPB4)

PDB	External Database	Details
Met -11	-	expression tag
Arg -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Asn 56	His 56	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	204898.3
Non-Polymers*	Number of molecules	64
	Total formula weight	4913.8
All*	Total formula weight	209812.1

*Water molecules are not included.