7KUG

Fe-S cluster-bound transcription activator WhiB7 in complex with the SigmaAr4-RNAP Beta flap tip chimera

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Probable transcriptional regulator WhiB7	polymer	79	8835.8	2	UniProt (Q6MX01) Pfam (PF02467)	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
2	B, D (B, D)	RNA polymerase sigma factor, DNA-directed RNA polymerase subunit beta chimera	polymer	112	12744.0	2	UniProt (A0A654TMB9) UniProt (A1KGE7) Pfam (PF04545) UniProt (by SIFTS) (P9WGI1) UniProt (by SIFTS) (P9WGY9)	Mycobacterium tuberculosis	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	E, F (A, C)	IRON/SULFUR CLUSTER	non-polymer		351.6	2	Chemie (SF4)
4	G, H, I, J (A, B, C, D)	water	water		18.0	333	Chemie (HOH)

Sequence modifications

A, C: 1 - 79 (UniProt: Q6MX01)

PDB	External Database	Details
Mse 27	Leu 27	engineered mutation
Mse 42	Leu 42	engineered mutation

B, D: 446 - 528 (UniProt: A0A654TMB9)

PDB	External Database	Details
Mse 438	-	initiating methionine
Ala 439	-	expression tag
His 440	-	expression tag
His 441	-	expression tag
His 442	-	expression tag
His 443	-	expression tag
His 444	-	expression tag
His 445	-	expression tag
Gly 529	-	linker
Ser 530	-	linker
Ser 531	-	linker
Gly 532	-	linker
Ser 533	-	linker
Gly 534	-	linker

B, D: 535 - 549 (UniProt: A1KGE7)

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	43159.6
Non-Polymers*	Number of molecules	2
	Total formula weight	703.3
All*	Total formula weight	43862.9

*Water molecules are not included.