7KR3

Human DNA Ligase 1(E346A/E592A) Bound to a bulged DNA substrate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA ligase 1	polymer	647	71916.2	1	UniProt (P18858) Pfam (PF04675) Pfam (PF01068) Pfam (PF04679) In PDB	Homo sapiens (Human)	DNA ligase I,Polydeoxyribonucleotide synthase [ATP] 1
2	B	DNA (5'-D(*AP*AP*TP*GP*TP*CP*TP*GP*CP*CP*CP*C)-3')	polymer	12	3598.4	1		Saccharomyces cerevisiae
3	C	DNA (5'-D(P*AP*TP*TP*CP*TP*GP*C)-3')	polymer	7	2088.4	1		Saccharomyces cerevisiae
4	D	DNA (5'-D(*GP*CP*AP*GP*AP*AP*TP*GP*GP*GP*CP*AP*GP*AP*CP*AP*TP*T)-3')	polymer	18	5589.6	1		Saccharomyces cerevisiae
5	C	ADENOSINE MONOPHOSPHATE	non-polymer		347.2	1	Chemie (AMP)
6	D	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
7		water	water		18.0	17	Chemie (HOH)

Sequence modifications

A: 262 - 904 (UniProt: P18858)

PDB	External Database	Details
Ser 258	-	expression tag
Asn 259	-	expression tag
Asp 260	-	expression tag
Met 261	-	expression tag
Ala 346	Glu 346	engineered mutation
Ala 592	Glu 592	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	83192.6
Non-Polymers*	Number of molecules	2
	Total formula weight	406.3
All*	Total formula weight	83598.9

*Water molecules are not included.