7KPC

Dihydrodipicolinate synthase (DHDPS) from C.jejuni, E88Q mutant with pyruvate bound in the active site and L-lysine bound at the allosteric site

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	4-hydroxy-tetrahydrodipicolinate synthase	polymer	310	34184.3	6	UniProt (Q9PPB4) Pfam (PF00701)	Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)	HTPA synthase
2	AB, CC, G, JA, RB... (D, F, A, C, E...)	LYSINE	non-polymer		147.2	6	Chemie (LYS)
3	AA, BB, DC, H, I... (B, D, F, A, C...)	MAGNESIUM ION	non-polymer		24.3	14	Chemie (MG)
4	BA, CB, DB, EB, EC... (B, D, F, C, A...)	ACETATE ION	non-polymer		59.0	15	Chemie (ACT)
5	AC, CA, DA, EA, FA... (E, B, D, F, C...)	1,2-ETHANEDIOL	non-polymer		62.1	28	Chemie (EDO)
6	BC, GA, LC, MB, NB... (E, B, F, D, C...)	TRIETHYLENE GLYCOL	non-polymer		150.2	10	Chemie (PGE)
7	HA, MC, NC, OC, PB... (B, F, D, C, A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	12	Chemie (PEG)
8	IA, PC, QB (B, F, D)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
9	QC, RC, SC, TC, UC... (A, B, C, D, E...)	water	water		18.0	1539	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 1 - 298 (UniProt: Q9PPB4)

PDB	External Database	Details
Met -11	-	expression tag
Arg -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Gln 88	Glu 88	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	205105.6
Non-Polymers*	Number of molecules	88
	Total formula weight	6898.5
All*	Total formula weight	212004.1

*Water molecules are not included.