7KN9

Dihydrodipicolinate synthase (DHDPS) from C.jejuni, E88A mutant with pyruvate bound in the active site and L-lysine bound at the allosteric site

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	4-hydroxy-tetrahydrodipicolinate synthase	polymer	310	34127.2	6	UniProt (Q9PPB4) Pfam (PF00701)	Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)	HTPA synthase
2	AA, AB, BA, BB, CA... (B, D, A, E, C...)	MAGNESIUM ION	non-polymer		24.3	25	Chemie (MG)
3	DA, DB, M, N, OB... (B, D, A, E, C...)	TRIETHYLENE GLYCOL	non-polymer		150.2	8	Chemie (PGE)
4	AC, BC, CC, DC, EA... (F, B, D, A, C...)	1,2-ETHANEDIOL	non-polymer		62.1	27	Chemie (EDO)
5	EC, FC, GC, IB, JA... (F, D, B, A, C)	ACETATE ION	non-polymer		59.0	12	Chemie (ACT)
6	HC, LA, V (F, B, A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	3	Chemie (PEG)
7	IC, KB, MA, TB, W... (F, D, B, E, A...)	LYSINE	non-polymer		147.2	6	Chemie (LYS)
8	JC, KC, LC, MC, NC... (A, B, C, D, E...)	water	water		18.0	1034	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 1 - 298 (UniProt: Q9PPB4)

PDB	External Database	Details
Met -11	-	expression tag
Arg -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ala 88	Glu 88	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	204763.3
Non-Polymers*	Number of molecules	81
	Total formula weight	5394.9
All*	Total formula weight	210158.2

*Water molecules are not included.