7KN2

Dihydrodipicolinate synthase (DHDPS) from C.jejuni, E88A mutant with pyruvate bound in the active site

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	4-hydroxy-tetrahydrodipicolinate synthase	polymer	310	34127.2	6	UniProt (Q9PPB4) Pfam (PF00701)	Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)	HTPA synthase
2	FA, G, H, MA, R... (E, A, F, B, C)	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)
3	CA, GA, I, S, X (D, E, A, B, C)	TRIETHYLENE GLYCOL	non-polymer		150.2	5	Chemie (PGE)
4	J, K (A)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5	AA, BA, DA, HA, IA... (C, D, E, A, F...)	1,2-ETHANEDIOL	non-polymer		62.1	18	Chemie (EDO)
6	P, PA, V (A, F, B)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	3	Chemie (PEG)
7	EA, Q (E, B)	ACETATE ION	non-polymer		59.0	2	Chemie (ACT)
8	QA, RA, SA, TA, UA... (A, B, C, D, E...)	water	water		18.0	397	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 1 - 298 (UniProt: Q9PPB4)

PDB	External Database	Details
Met -11	-	expression tag
Arg -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ala 88	Glu 88	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	204763.3
Non-Polymers*	Number of molecules	36
	Total formula weight	2634.6
All*	Total formula weight	207397.9

*Water molecules are not included.