7KML

cryo-EM structure of SARS-CoV-2 spike in complex with Fab 15033-7, three RBDs bound

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Spike glycoprotein	polymer	1274	141170.3	3	UniProt (P0DTC2) Pfam (PF16451) Pfam (PF09408) Pfam (PF19209) Pfam (PF01601)	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)	S glycoprotein,E2,Peplomer protein
2	D, F, H (L, M, N)	Fab 15033-7 light chain	polymer	214	23366.9	3		Homo sapiens
3	E, G, I (H, I, J)	Fab 15033-7 heavy chain	polymer	225	23513.3	3		Homo sapiens
4	AA, BA, CA, FA, GA... (a, b, c, f, g...)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	21	In PDB GlyTouCan (G42666HT)
5	DA, EA, L, M, U... (d, e, F, G, U...)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	6	In PDB GlyTouCan (G15407YE)
6	AB, BB, KA, LA, MA... (C, A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	18	Chemie (NAG)

Sequence modifications

A, B, C: 1 - 1211 (UniProt: P0DTC2)

PDB	External Database	Details
Ser 682	Arg 682	conflict
Ser 683	Arg 683	conflict
Ser 685	Arg 685	conflict
Pro 817	Phe 817	conflict
Pro 892	Ala 892	conflict
Pro 899	Ala 899	conflict
Pro 942	Ala 942	conflict
Pro 986	Lys 986	conflict
Pro 987	Val 987	conflict
Ser 1212	-	expression tag
Gly 1213	-	expression tag
Gly 1214	-	expression tag
Tyr 1215	-	expression tag
Ile 1216	-	expression tag
Pro 1217	-	expression tag
Glu 1218	-	expression tag
Ala 1219	-	expression tag
Pro 1220	-	expression tag
Arg 1221	-	expression tag
Asp 1222	-	expression tag
Gly 1223	-	expression tag
Gln 1224	-	expression tag
Ala 1225	-	expression tag
Tyr 1226	-	expression tag
Val 1227	-	expression tag
Arg 1228	-	expression tag
Lys 1229	-	expression tag
Asp 1230	-	expression tag
Gly 1231	-	expression tag
Glu 1232	-	expression tag
Trp 1233	-	expression tag
Val 1234	-	expression tag
Leu 1235	-	expression tag
Leu 1236	-	expression tag
Ser 1237	-	expression tag
Thr 1238	-	expression tag
Phe 1239	-	expression tag
Leu 1240	-	expression tag
Asn 1241	-	expression tag
Ser 1242	-	expression tag
Gly 1243	-	expression tag
Arg 1244	-	expression tag
Ala 1245	-	expression tag
His 1246	-	expression tag
His 1247	-	expression tag
His 1248	-	expression tag
His 1249	-	expression tag
His 1250	-	expression tag
His 1251	-	expression tag
Gly 1252	-	expression tag
Ala 1253	-	expression tag
Gly 1254	-	expression tag
Gly 1255	-	expression tag
Leu 1256	-	expression tag
Asn 1257	-	expression tag
Asp 1258	-	expression tag
Ile 1259	-	expression tag
Phe 1260	-	expression tag
Glu 1261	-	expression tag
Ala 1262	-	expression tag
Gln 1263	-	expression tag
Lys 1264	-	expression tag
Ile 1265	-	expression tag
Glu 1266	-	expression tag
Trp 1267	-	expression tag
His 1268	-	expression tag
Glu 1269	-	expression tag
Asp 1270	-	expression tag
Thr 1271	-	expression tag
Ala 1272	-	expression tag
Ala 1273	-	expression tag
Ala 1274	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	564151.2
Branched	Number of molecules	27
	Total formula weight	12431.7
Non-Polymers*	Number of molecules	18
	Total formula weight	3981.7
All*	Total formula weight	580564.6

*Water molecules are not included.