7KMF

Sugar phosphate activation of the stress sensor eIF2B

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (C, D)	Translation initiation factor eIF-2B subunit beta	polymer	367	41049.6	2	UniProt (P49770) Pfam (PF01008)	Homo sapiens (Human)	S20I15,S20III15,eIF-2B GDP-GTP exchange factor subunit beta
2	C, D (F, E)	Translation initiation factor eIF-2B subunit delta	polymer	523	57640.2	2	UniProt (Q9UI10) Pfam (PF01008)	Homo sapiens (Human)	eIF-2B GDP-GTP exchange factor subunit delta
3	E, F (H, G)	Translation initiation factor eIF-2B subunit alpha	polymer	377	40552.9	2	UniProt (Q14232) Pfam (PF01008)	Homo sapiens (Human)	eIF-2B GDP-GTP exchange factor subunit alpha
4	G, H (B, I)	Translation initiation factor eIF-2B subunit epsilon	polymer	721	80466.6	2	UniProt (Q13144) Pfam (PF02020)	Homo sapiens (Human)	eIF-2B GDP-GTP exchange factor subunit epsilon
5	I, J (J, K)	Translation initiation factor eIF-2B subunit gamma	polymer	452	50304.2	2	UniProt (Q9NR50) Pfam (PF00483)	Homo sapiens (Human)	eIF-2B GDP-GTP exchange factor subunit gamma
6	K, L (H, G)	6-O-phosphono-beta-D-fructofuranose	non-polymer		260.1	2	Chemie (F6P)
7	M, N, O, P, Q... (C, D, F, E, H...)	water	water		18.0	18	Chemie (HOH)

Sequence modifications

C, D: 1 - 351 (UniProt: P49770)

PDB	External Database	Details
Met -15	-	initiating methionine
Asp -14	-	expression tag
Tyr -13	-	expression tag
Lys -12	-	expression tag
Asp -11	-	expression tag
Asp -10	-	expression tag
Asp -9	-	expression tag
Asp -8	-	expression tag
Lys -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Ser 0	-	expression tag

H, G: 1 - 305 (UniProt: Q14232)

PDB	External Database	Details
Gly 306	-	expression tag
Gly 307	-	expression tag
Glu 308	-	expression tag
Asn 309	-	expression tag
Leu 310	-	expression tag
Tyr 311	-	expression tag
Phe 312	-	expression tag
Gln 313	-	expression tag
Ala 314	-	expression tag
Glu 315	-	expression tag
Asp 316	-	expression tag
Lys 317	-	expression tag
Gly 318	-	expression tag
Gly 319	-	expression tag
Gly 320	-	expression tag
Ser 321	-	expression tag
Gly 322	-	expression tag
Gly 323	-	expression tag
Gly 324	-	expression tag
Gly 325	-	expression tag
Ser 326	-	expression tag
Gly 327	-	expression tag
Gly 328	-	expression tag
Gly 329	-	expression tag
Gly 330	-	expression tag
Ser 331	-	expression tag
Ala 332	-	expression tag
Ser 333	-	expression tag
Gln 334	-	expression tag
Gly 335	-	expression tag
Gly 336	-	expression tag
Leu 337	-	expression tag
Asn 338	-	expression tag
Asp 339	-	expression tag
Ile 340	-	expression tag
Phe 341	-	expression tag
Glu 342	-	expression tag
Ala 343	-	expression tag
Gln 344	-	expression tag
Lys 345	-	expression tag
Ile 346	-	expression tag
Glu 347	-	expression tag
Trp 348	-	expression tag
His 349	-	expression tag
Glu 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Gly 353	-	expression tag
Gly 354	-	expression tag
Ser 355	-	expression tag
Gly 356	-	expression tag
Gly 357	-	expression tag
Gly 358	-	expression tag
Gly 359	-	expression tag
Ser 360	-	expression tag
Gly 361	-	expression tag
Gly 362	-	expression tag
Gly 363	-	expression tag
Gly 364	-	expression tag
Ser 365	-	expression tag
Gly 366	-	expression tag
Arg 367	-	expression tag
Asp 368	-	expression tag
Gln 369	-	expression tag
Asp 370	-	expression tag
Tyr 371	-	expression tag
Lys 372	-	expression tag
Asp 373	-	expression tag
Asp 374	-	expression tag
Asp 375	-	expression tag
Asp 376	-	expression tag
Lys 377	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	540027.2
Non-Polymers*	Number of molecules	2
	Total formula weight	520.3
All*	Total formula weight	540547.4

*Water molecules are not included.