7KLY

Dihydrodipicolinate synthase (DHDPS) from C.jejuni, H59N mutant with pyruvate bound in the active site and L-lysine bound at the allosteric site

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	4-hydroxy-tetrahydrodipicolinate synthase	polymer	310	34161.2	6	UniProt (Q9PPB4) Pfam (PF00701)	Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)	HTPA synthase
2	G, GA, MB, S, XA... (A, C, E, B, D...)	LYSINE	non-polymer		147.2	6	Chemie (LYS)
3	H, HA, NB, T, YA... (A, C, E, B, D...)	TETRAETHYLENE GLYCOL	non-polymer		194.2	6	Chemie (PG4)
4	AB, AC, BB, BC, CB... (D, F, A, C, E...)	1,2-ETHANEDIOL	non-polymer		62.1	36	Chemie (EDO)
5	FB, GB, GC, HB, HC... (D, F, A, C, E...)	ACETATE ION	non-polymer		59.0	12	Chemie (ACT)
6	AA, BA, CA, DA, EA... (B, D, A, C)	MAGNESIUM ION	non-polymer		24.3	10	Chemie (MG)
7	FA, JC, KB, R (B, F, D, A)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
8	IB, IC, TA, UA, WB... (D, F, C, E, B)	TRIETHYLENE GLYCOL	non-polymer		150.2	6	Chemie (PGE)
9	LB, WA (D, C)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
10	KC, LC, MC, NC, OC... (A, B, C, D, E...)	water	water		18.0	957	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 1 - 298 (UniProt: Q9PPB4)

PDB	External Database	Details
Met -11	-	expression tag
Arg -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Asn 59	His 59	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	204967.3
Non-Polymers*	Number of molecules	82
	Total formula weight	6716.2
All*	Total formula weight	211683.5

*Water molecules are not included.