7KKD

Dihydrodipicolinate synthase (DHDPS) from C.jejuni, N84A mutant with pyruvate bound in the active site and R,R-bislysine bound at the allosteric site

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	4-hydroxy-tetrahydrodipicolinate synthase	polymer	310	34142.2	6	UniProt (Q9PPB4) Pfam (PF00701)	Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)	HTPA synthase
2	AA, G, O (C, A, B)	(2R,5R)-2,5-diamino-2,5-bis(4-aminobutyl)hexanedioic acid	non-polymer		318.4	3	Chemie (3VN)
3	BA, FA, GA, H, OA... (C, D, A, F, B)	MAGNESIUM ION	non-polymer		24.3	9	Chemie (MG)
4	CA, DA, HA, I, J... (C, D, A, E, F...)	ACETATE ION	non-polymer		59.0	10	Chemie (ACT)
5	EA, K, KA, L, LA... (C, A, E, F, B)	1,2-ETHANEDIOL	non-polymer		62.1	10	Chemie (EDO)
6	IA, M, MA, SA, Z (D, A, E, F, B)	TRIETHYLENE GLYCOL	non-polymer		150.2	5	Chemie (PGE)
7	N, NA (A, E)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
8	TA, UA, VA, WA, XA... (A, B, C, D, E...)	water	water		18.0	1832	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 1 - 298 (UniProt: Q9PPB4)

PDB	External Database	Details
Met -11	-	expression tag
Arg -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ala 84	Asn 84	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	204853.4
Non-Polymers*	Number of molecules	39
	Total formula weight	3348.2
All*	Total formula weight	208201.6

*Water molecules are not included.