7KAY

Crystal structure of OhyA(E82A)-18:1 complex from Staphylococcus aureus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Oleate hydratase	polymer	611	69834.7	3	UniProt (A0A0D6GJV1) Pfam (PF06100)	Staphylococcus aureus	Myosin-cross-reactive antigen
2	D, G, J (A, B, C)	OLEIC ACID	non-polymer		282.5	3	Chemie (OLA)
3	E, H (A, B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4	F, I (A, B)	POTASSIUM ION	non-polymer		39.1	2	Chemie (K)
5	K, L, M (A, B, C)	water	water		18.0	2167	Chemie (HOH)

Sequence modifications

A, B, C: 1 - 591 (UniProt: A0A0D6GJV1)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 82	Glu 82	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	209504.1
Non-Polymers*	Number of molecules	7
	Total formula weight	1109.8
All*	Total formula weight	210613.8

*Water molecules are not included.