7KAP

Cryo-EM structure of the Sec complex from S. cerevisiae, Sec61 pore mutant, class with Sec62, conformation 1 (C1)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Protein transport protein SEC61	polymer	480	52936.1	1	UniProt (P32915) Pfam (PF10559) Pfam (PF00344)	Saccharomyces cerevisiae BY4741	Sec61 complex subunit SEC61,Sec61 complex subunit alpha
2	B (C)	Protein transport protein SSS1	polymer	80	8958.6	1	UniProt (P35179) Pfam (PF00584)	Saccharomyces cerevisiae BY4741	Sec61 complex subunit SSS1,Sec61 complex subunit gamma,Ssh1 complex subunit SSS1,Ssh1 complex subunit gamma
3	C (B)	Protein transport protein SBH1	polymer	82	8723.2	1	UniProt (P52870) Pfam (PF03911)	Saccharomyces cerevisiae BY4741	Sec61 complex subunit SBH1,Sec61 complex subunit beta
4	D (D)	Protein translocation protein SEC63	polymer	694	77933.7	1	UniProt (P14906) Pfam (PF00226)	Saccharomyces cerevisiae BY4741	Protein NPL1,Sec62/63 complex 73 kDa subunit
5	E (E)	Translocation protein SEC66	polymer	206	24263.9	1	UniProt (P33754) Pfam (PF09802)	Saccharomyces cerevisiae BY4741	Protein HSS1,Sec62/63 complex 31.5 kDa subunit
6	F (F)	Translocation protein SEC72	polymer	193	21631.1	1	UniProt (P39742)	Saccharomyces cerevisiae BY4741	Sec62/63 complex 23 kDa subunit,p23
7	G (G)	Protein transport protein Sec62	polymer	56	4783.9	1		Saccharomyces cerevisiae BY4741

Sequence modifications

A: 1 - 480 (UniProt: P32915)

PDB	External Database	Details
Leu 90	Met 90	variant
Ile 185	Thr 185	variant
Ile 294	Met 294	variant
Leu 450	Met 450	variant

D: 2 - 663 (UniProt: P14906)

PDB	External Database	Details
Gly -13	-	expression tag
Gly -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Gly -6	-	expression tag
Ser -5	-	expression tag
Gly -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag
Ala 664	-	expression tag
Gly 665	-	expression tag
Gly 666	-	expression tag
Ala 667	-	expression tag
Thr 668	-	expression tag
Thr 669	-	expression tag
Ala 670	-	expression tag
Ser 671	-	expression tag
Gly 672	-	expression tag
Thr 673	-	expression tag
Gly 674	-	expression tag
Glu 675	-	expression tag
Asn 676	-	expression tag
Leu 677	-	expression tag
Tyr 678	-	expression tag
Phe 679	-	expression tag
Gln 680	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	199230.5
All*	Total formula weight	199230.5

*Water molecules are not included.