7KAO
Cryo-EM structure of the Sec complex from S. cerevisiae, Sec61 pore mutant, class without Sec62
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Protein transport protein SEC61 | polymer | 480 | 52936.1 | 1 | UniProt (P32915) Pfam (PF10559) Pfam (PF00344) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Sec61 complex subunit SEC61,Sec61 complex subunit alpha |
| 2 | B (C) | Protein transport protein SSS1 | polymer | 80 | 8958.6 | 1 | UniProt (P35179) Pfam (PF00584) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Sec61 complex subunit SSS1,Sec61 complex subunit gamma,Ssh1 complex subunit SSS1,Ssh1 complex subunit gamma |
| 3 | C (B) | Protein transport protein SBH1 | polymer | 82 | 8723.2 | 1 | UniProt (P52870) Pfam (PF03911) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Sec61 complex subunit SBH1,Sec61 complex subunit beta |
| 4 | D (D) | Protein translocation protein SEC63 | polymer | 662 | 75301.1 | 1 | UniProt (P14906) Pfam (PF00226) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Protein NPL1,Sec62/63 complex 73 kDa subunit |
| 5 | E (E) | Translocation protein SEC66 | polymer | 206 | 24263.9 | 1 | UniProt (P33754) Pfam (PF09802) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Protein HSS1,Sec62/63 complex 31.5 kDa subunit |
| 6 | F (F) | Translocation protein SEC72 | polymer | 193 | 21631.1 | 1 | UniProt (P39742) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Sec62/63 complex 23 kDa subunit,p23 |
Sequence modifications
A: 1 - 480 (UniProt: P32915)
| PDB | External Database | Details |
|---|---|---|
| Leu 90 | Met 90 | engineered mutation |
| Ile 185 | Thr 185 | engineered mutation |
| Ile 294 | Met 294 | engineered mutation |
| Leu 450 | Met 450 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 191814.0 | |
| All* | Total formula weight | 191814.0 |
